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Articles 1 - 7 of 7
Full-Text Articles in Biology
The Effect Of The Zika Virus On Rna Stress Granule Components, Nina Williams
The Effect Of The Zika Virus On Rna Stress Granule Components, Nina Williams
Biological Sciences
In recent years, Zika virus (ZIKV) has taken over mainstream media. It captivated the world with the images of microcephaly babies born to infected mothers and the appearance of Guillain-Barré syndrome emerging from infected adults. ZIKV is transmitted by the Aedes mosquito. This virus is composed of a single-stranded positive-sense RNA genome that belongs to the Flaviviridae family. Our long-term goal is to understand the mechanisms in which the virus subverts the host organism’s translation machinery by modulation of RNA stress granules (SGs). Stress granules are RNA-protein complexes found in the cytoplasm that form when the cell is exposed to …
The Effects Of Intrahippocampal Estradiol Administration On Spatial Memory And Protein Expression In Rats Fed A High Fat Diet, Lauren M. Maitner
The Effects Of Intrahippocampal Estradiol Administration On Spatial Memory And Protein Expression In Rats Fed A High Fat Diet, Lauren M. Maitner
Biological Sciences
Estradiol acts throughout the body; one key target that expresses large numbers of estradiol receptors is the brain, and specifically the hippocampus. Estradiol can exhibit neuroprotective effects in the brain. However, the set of pathways through which this occurs is not well understood. In vitro work has shown that administration of estradiol to hippocampal neurons inactivates caspase 3, a protease involved in apoptosis and the cleavage of tau. Cleavage of tau results in the formation of neurofibrillary tangles. Together with formation of amyloid-beta plaques, this is one of the hallmarks of Alzheimer’s disease. We tested the hypothesis that administration of …
Development Of A Rapid Small-Scale Purification Method For The Quantitation Of Heparin-Like Glycosaminoglycans From Cell Culture Media, Marina Danielle Infantado
Development Of A Rapid Small-Scale Purification Method For The Quantitation Of Heparin-Like Glycosaminoglycans From Cell Culture Media, Marina Danielle Infantado
Biological Sciences
Heparin is an anticoagulant medicinally used to inhibit blood clotting. It is commonly administered to patients requiring surgery or kidney dialysis. Presently, it is produced from animal tissues, but a recent contamination crisis pointed to the need for a safer source of the drug. Our project seeks to develop a rapid, inexpensive, high-throughput assay to quantitate the production of heparin and other glycosaminoglycans (GAGs) from cultured mammalian cells. In order to quantitate heparin from cell culture media, a purification method is needed to separate GAGs from interfering constituents in the media. We developed a purification protocol that absorbs Pluronic and …
The Role Of Rck/P54 Enzymatic Activity During Hepatitis C Infection, Rachel E. Eager
The Role Of Rck/P54 Enzymatic Activity During Hepatitis C Infection, Rachel E. Eager
Biological Sciences
Hepatitis C virus (HCV) infected 30,500 people in the United States alone in 2014. As a positive-sense single-stranded RNA virus, the HCV genome facilitates translation and replication of the virus within the host cell. During infection, HCV subverts numerous host proteins to aid in viral production. One such protein is DDX6, a DEAD-box helicase that is associated with the decapping complex and microRNA Induced Silencing Complex (miRISC). Previous studies in our lab have shown that DDX6 is required for HCV expression. Specifically, DDX6 modulates the interaction of miR-122 with the HCV 5’ UTR and promotes stability and replication of the …
Effects Of Zinc On Cognition, Anthony Piche
Effects Of Zinc On Cognition, Anthony Piche
Biological Sciences
Insulin resistance is the defining symptom of type 2 diabetes and a key risk factor for Alzheimer’s disease. Work from our lab and others has shown that insulin is a key component of brain pathways mediating cognition; specifically, mechanisms that transduce hippocampal memory processing. In the hippocampus, insulin regulates glucose metabolism through translocation of the glucose transporter GluT4 and modulates several other molecular cascades. Studies where insulin is studied as a cognitive modulator have generally used a formulation that contains zinc to stabilize insulin. However, zinc itself modulates molecular signaling pathways including AKT and GSK3β, which are both downstream of …
One-Step Cellular Micro-Rna Detection With Programmable Dna Nanoswitches, Molly F. Macisaac
One-Step Cellular Micro-Rna Detection With Programmable Dna Nanoswitches, Molly F. Macisaac
Biological Sciences
MicroRNAs play important roles in gene regulation. Additionally, differential expression of specific microRNAs have been correlated with a wide range of diseases. Sensitive and selective detection of microRNAs is thus important for enabling their use as biomarkers, drugs, or drug targets. Current detection techniques such as northern blotting and quantitative real-time PCR require skilled personnel and expensive equipment to execute complex and time consuming assays. Here we develop and validate a one-step, non-enzymatic microRNA detection assay using DNA nanoswitches programmed to recognize and bind a specific microRNA. Binding induces a loop in the structure, allowing the target microRNA to be …
Bruton's Tyrosine Kinase Is A Potential Therapeutic Target In Non-Small Cell Lung Cancer Cells, Abdul Manan Sanni-Adam
Bruton's Tyrosine Kinase Is A Potential Therapeutic Target In Non-Small Cell Lung Cancer Cells, Abdul Manan Sanni-Adam
Biological Sciences
No abstract provided.