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Chronic Arsenic Exposure In Nanomolar Concentrations Accelerates Senescent Phenotypes In Vitro, Heather M. Sabo

Biological Sciences

Arsenic is recognized as an environmental carcinogen, in which over 100 million individuals worldwide experience chronic exposure through contaminated drinking water. Arsenic exposure is linked to several health conditions, such as cancer, diabetes, skin lesions, immune dysfunction, and cardiovascular disease. Here we test the hypothesis that toxicants, such as arsenic, accelerate the program of senescence. Human (IMR-90) primary diploid fibroblasts were chronically exposed to arsenic at nanomolar concentrations and the impact on the expression of senescent-associated mRNAs was evaluated. IMR-90 cells were exposed either to untreated media or exposed to 130nM and 330nM AsCl3 supplemented media. RNA samples were extracted …