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The Proteomic Response Of Sea Squirts (Genus Ciona) To Acute Heat Stress: A Global Perspective On The Thermal Stability Of Proteins, Loredana Serafini, Jay B. Hann, Dietmar Kültz, Lars Tomanek

Biological Sciences

Congeners belonging to the genus Ciona have disparate distributions limited by temperature. Ciona intestinalis is more widespread with a cosmopolitan distribution ranging from tropical to sub-arctic zones, while Ciona savignyi is limited to temperate-latitudes of the northern Pacific Ocean. To compare the heat stress response between congeners, we quantified changes in protein expression using proteomics. Animals were exposed to 22 °C, 25 °C, and 28 °C for 6 h, then recovered at a control temperature (13 °C) for 16 h (high heat stress experiment). In a second experiment we exposed animals to lower levels of heat stress at 18 °C, …