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Cyclic Di-Gmp Phosphodiesterases Rmda And Rmdb Are Involved In Regulating Colony Morphology And Development In Streptomyces Coelicolor. Journal Of Bacteriology, Travis D. Hull, Min-Hyung Ryu, Matthew J. Sullivan, Ryan C. Johnson, Nikola T. Klena, Robert M. Gelger, Mark Gomelsky, Jennifer A. Bennett
Cyclic Di-Gmp Phosphodiesterases Rmda And Rmdb Are Involved In Regulating Colony Morphology And Development In Streptomyces Coelicolor. Journal Of Bacteriology, Travis D. Hull, Min-Hyung Ryu, Matthew J. Sullivan, Ryan C. Johnson, Nikola T. Klena, Robert M. Gelger, Mark Gomelsky, Jennifer A. Bennett
Biology and Earth Science Faculty Scholarship
Cyclic dimeric GMP (c-di-GMP) regulates numerous processes in Gram-negative bacteria, yet little is known about its role in Gram-positive bacteria. Here we characterize two c-di-GMP phosphodiesterases from the filamentous high-GC Gram-positive actinobacterium Streptomyces coelicolor, involved in controlling colony morphology and development. A transposon mutation in one of the two phosphodiesterase genes, SCO0928, hereby designated rmdA (regulator ofmorphology and development A), resulted in decreased levels of spore-specific gray pigment and a delay in spore formation. The RmdA protein contains GGDEF-EAL domains arranged in tandem and possesses c-di-GMP phosphodiesterase activity, as is evident from in vitro enzymatic assays using the purified protein. …