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Nova Southeastern University

Bacterial Artificial Chromosome

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Biology

A Bac-Based Physical Map Of The Hessian Fly Genome Anchored To Polytene Chromosomes, Rajat Aggarwal, Thiago R. Benatti, Navdeep Gill, Chaoyang Zhao, Ming-Shun Chen, John P. Fellers, Brandon J. Schemerhorn, Jeff J. Stuart Jul 2009

A Bac-Based Physical Map Of The Hessian Fly Genome Anchored To Polytene Chromosomes, Rajat Aggarwal, Thiago R. Benatti, Navdeep Gill, Chaoyang Zhao, Ming-Shun Chen, John P. Fellers, Brandon J. Schemerhorn, Jeff J. Stuart

Biology Faculty Articles

Background: The Hessian fly (Mayetiola destructor) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results: An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC) clones corresponding …


Construction, Alignment And Analysis Of Twelve Framework Physical Maps That Represent The Ten Genome Types Of The Genus Oryza, Hyeran Kim, Bonnie Hurwitz, Yeisoo Yu, Kristi Collura, Navdeep Gill, Phillip Sanmiguel, James C. Mullikin, Christopher Maher, William Nelson, Marina Wissotski, Michele Braidotti, David Kudrna, José Luis Goicoechea, Lincoln Stein, Doreen Ware, Scott A. Jackson, Carol Soderlund, Rod A. Wing Feb 2008

Construction, Alignment And Analysis Of Twelve Framework Physical Maps That Represent The Ten Genome Types Of The Genus Oryza, Hyeran Kim, Bonnie Hurwitz, Yeisoo Yu, Kristi Collura, Navdeep Gill, Phillip Sanmiguel, James C. Mullikin, Christopher Maher, William Nelson, Marina Wissotski, Michele Braidotti, David Kudrna, José Luis Goicoechea, Lincoln Stein, Doreen Ware, Scott A. Jackson, Carol Soderlund, Rod A. Wing

Biology Faculty Articles

We describe the establishment and analysis of a genus-wide comparative framework composed of 12 bacterial artificial chromosome fingerprint and end-sequenced physical maps representing the 10 genome types of Oryza aligned to the O. sativa ssp. japonica reference genome sequence. Over 932 Mb of end sequence was analyzed for repeats, simple sequence repeats, miRNA and single nucleotide variations, providing the most extensive analysis of Oryza sequence to date.


Integration Of Hybridization-Based Markers (Overgos) Into Physical Maps For Comparative And Evolutionary Explorations In The Genus Oryza And In Sorghum, Barbara L. Hass-Jacobus, Montona Futrell-Griggs, Brian Abernathy, Rick Westerman, Jose-Luis Goicoechea, Joshua Stein, Patricia Klein, Bonnie Hurwitz, Bin Zhou, Fariborz Rakhshan, Abhijit Sanyal, Navdeep Gill, Jer-Young Lin, Jason G. Walling, Mei Zhong Luo, Jetty Siva S Ammiraju, Dave Kudrna, Hye Ran Kim, Doreen Ware, Rod A. Wing, Phillip San Miguel, Scott A. Jackson Aug 2006

Integration Of Hybridization-Based Markers (Overgos) Into Physical Maps For Comparative And Evolutionary Explorations In The Genus Oryza And In Sorghum, Barbara L. Hass-Jacobus, Montona Futrell-Griggs, Brian Abernathy, Rick Westerman, Jose-Luis Goicoechea, Joshua Stein, Patricia Klein, Bonnie Hurwitz, Bin Zhou, Fariborz Rakhshan, Abhijit Sanyal, Navdeep Gill, Jer-Young Lin, Jason G. Walling, Mei Zhong Luo, Jetty Siva S Ammiraju, Dave Kudrna, Hye Ran Kim, Doreen Ware, Rod A. Wing, Phillip San Miguel, Scott A. Jackson

Biology Faculty Articles

Background: With the completion of the genome sequence for rice (Oryza sativa L.), the focus of rice genomics research has shifted to the comparison of the rice genome with genomes of other species for gene cloning, breeding, and evolutionary studies. The genus Oryza includes 23 species that shared a common ancestor 8–10 million years ago making this an ideal model for investigations into the processes underlying domestication, as many of the Oryza species are still undergoing domestication. This study integrates high-throughput, hybridization-based markers with BAC end sequence and fingerprint data to construct physical maps of rice chromosome 1 orthologues in …