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Mutational Studies In The Dntp Binding Pocket Of Human Immunodeficiency Virus Type 1 Reverse Transcriptase, Smita Shukla
Mutational Studies In The Dntp Binding Pocket Of Human Immunodeficiency Virus Type 1 Reverse Transcriptase, Smita Shukla
Theses
It was earlier postulated that Gln 91 of HIV-1 RT stabilizes the side chain of Tyr 183 via hydrogen bonding interaction between 0(H) of Y1 83 and CO of Q91 (Harris et al., BIOCHEMISTRY 37: 9630, 1998). In an attempt to understand the function of Gln 91 in the catalytic mechanism, mutants of this residue (Gln91 - -> Ala and Gln91 - ->Asn) were generated and subjected to an in-depth analysis. The efficiency of reverse transcription of natural U5-PBS HIV-1 RNA template was severely impaired by both conservative and non-conservative substitutions from Gln—>Asn and Gln—> Ala, a result …