Biology Commons^™

Seasonal Resource Allocation And Accumulated Degree Day Estimation For Cuban Bulrush (Oxycarum Cubense) In The Southeastern U.S., Allison Squires

All Graduate Theses, Dissertations, and Other Capstone Projects

Cuban bulrush (Oxycaryum cubense (Poepp. & Kunth) Lye) is a non-native aquatic plant from South America and the West Indies, first introduced into the United States in 1878. This study focused on the resource allocation and accumulated degree day modeling of the monocephalous and polycephalous Cuban bulrush populations in Mississippi, Louisiana, and Florida. Environmental factors such as photoperiod and temperature were analyzed on its effect on plant growth. From October 2021- September 2022 Cuban bulrush biomass was harvested monthly from two 0.04 Ha (0.1 ac) plots in each state. Height was measured during each monthly event. Environmental monitoring of photoperiod …

Seasonal Starch Allocation In Starry Stonewort (Nitellopsis Obtusa) Harvested From Lake Koronis, Mn, And Development Of Non-Chemical Management Recommendations, Alyssa M. Haram

All Graduate Theses, Dissertations, and Other Capstone Projects

Starry stonewort (Nitellopsis obtusa) (Desvaux in Loiseleur) J. Groves is an invasive macro alga that can take over entire water columns and outcompete native species. Previous research has quantified seasonal life history and phenology but there is no research quantifying carbohydrate allocation which was the aim for Chapter II. Management efforts to control Nitellopsis obtusa have been limited to stressing the thalli and have not been able to directly target the reproductive bulbils on larger scales. Smaller scale efforts such as the use of hand pulling can be used but hand pulling is not realistic for larger infestations. Chapter III …

"Effects Of Chronic Clothianidin Exposure On Various Aspects Of Oncorhynchus Mykiss (Rainbow Trout) Development", Jacob Schmid

All Graduate Theses, Dissertations, and Other Capstone Projects

Agricultural run-off is one of the leading sources of environmental pollution in the United States. One major pollutant within this is insecticides, and among these, neonicotinoids which have become the most used insecticide class across the world. Neonicotinoids act as an agonist on the nicotinic acetylcholine receptor (nAChR) in the central nervous system of insects. Neonicotinoids are perceived to have low toxicity on non-target organisms however recently extensive literature has been published showing various effects on non-target organisms. Clothianidin is one of the most applied compounds belonging to the neonicotinoid class, yet few studies have investigated its chronic effects on …

The Role Of Insulin-Like Growth Factor I In The Development Of The Cerebellum In Euthyroid And Hypothyroid Mice, Prathibha Mangedarage

All Graduate Theses, Dissertations, and Other Capstone Projects

Thyroid hormone deficiency during development is known to cause anatomical defects in the cerebellum that lead to neurological deficits. It has been observed that insufficient thyroid hormone levels also decrease the levels of brain derived Insulin-like growth factor 1 (Igf-1). Given that the deficits caused by low Igf-1 levels are similar to those caused by low thyroid hormone, we designed this study to investigate if the deficits caused in the development of the cerebellum by low thyroid hormone levels may be due to the lack of sufficient Igf-1. We also sought to determine if ectopic over expression of Igf-1 in …

Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang

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Cytophaga hutchinsonii is a soil bacterium that can utilize cellulose as a carbon source. The cellulose utilization system of C. hutchinsonii has been predicted to be novel and its endoglucanases could potentially be used in the production of cellulosic based biofuels. These enzymes could be used to process cellulose to glucose or other soluble sugars, which can be further converted to ethanol by yeast fermentation. Current biofuels utilize starches, primarily from corn, which has direct competition with farming space for food crops. This study focuses on observing the growth of C. hutchinsonii knockout mutant strains on cellulose substrate to better …

Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver

All Graduate Theses, Dissertations, and Other Capstone Projects

Phenology is the study of critical life stages in plants in relation to shifts in environmental factors during seasonal changes. Starry stonewort is a green macro alga in the family Characeae native to Eurasia. Much of the Midwestern United States has been invaded by this species. Starry stonewort has been seen to have late season growth patterns in its invaded range. This study found that this growth pattern was dependent upon water temperature and light transmittance both of which affect biomass production and senescence. It was hypothesized that peak biomass would occur in the late summer (August), however we observed …

Effect Of Process-Oriented Guided-Inquiry Learning (Pogil) On Preservice Elementary Teachers' Understanding Of Biological Classification, Mackenzie Volz

All Graduate Theses, Dissertations, and Other Capstone Projects

This study examined preservice elementary teachers’ biological classification conceptions and whether process-oriented guided-inquiry learning (POGIL) or traditional pedagogies affect preservice elementary teachers’ understanding of biological classification conceptions. A literature review was completed to determine common biological classification conceptions that exist among all researched populations, what misconceptions are present regarding biological classification, and whether POGIL was an appropriate pedagogy to use in the experimental study. The findings from the literature review were used to develop a mixed-method research study. Both quantitative and qualitative data was gathered through the use of pre- and posttests and post-instruction clinical interviews. Participants (n = 47) …

Patterns Of Trematode Distribution From Hosts Collected At Lake Winnibigoshish, Minnesota, Scott Malotka

All Graduate Theses, Dissertations, and Other Capstone Projects

A comparative and seasonal study was performed on trematode populations and communities within waterfowl definitive hosts in order to determine important factors in the structuring of these communities. Furthermore, larval trematode populations were also sampled in order to compare larval stages found within snail hosts to adult stages found within collected waterfowl in order to determine where infections could have occurred with the trematode species. 52 birds including 18 blue-winged teal (Anas discors), 5 bufflehead (Bucephala albeola), 4 common goldeneye (Bucephala clangula), 1 greater scaup (Aythya marila), 1 green-winged teal (Anas carolinensis), 11 lesser scaup (Aythya affinis), 1 northern pintail …

Designing A Luminescence Assay To Measure In Vitro Binding Of The Fibronectin Attachment Protein (Fap) Of Mycobacterium Avium Subspecies Paratuberculosis To Fibronectin, Sirri Ngwa

All Graduate Theses, Dissertations, and Other Capstone Projects

The attachment and penetration of epithelial cells by Mycobacterium avium subsp. paratuberculosis (MAP) requires a bacterial fibronectin (FN) attachment protein FAP-P, which facilitates binding of FN by MAP. The region of FAP-P that interacts with FN is known, however, the region of FN that is bound by FAP-P is to be determined. Identification of the region of FN to which FAP-P binds would enable the synthesis of a competing peptide that could prevent FN binding by MAP. The first step toward locating this binding site is the design of a reliable FN binding assay. The purpose of this investigation was …

The Effects Of Linoleic Acid On Rubidium Uptake By Sodium Potassium Pumps In Rat Myocardial Cells, Mohammed Ahmed Gaafarelkhalifa

All Graduate Theses, Dissertations, and Other Capstone Projects

Congestive Heart failure is amongst the leading causes of death in the United States. It is treated through a variety of different therapeutic targets including the inhibition of sodium potassium pumps. Drugs currently used in this pathway of treatment such as Digitalis have adverse toxic effects. This study examines the inhibitory effects of linoleic acid (L.A) on sodium potassium pumps in isolated rat cardiomyocytes using the incorporation of rubidium detected by SEM/EDS technology as the index for sodium-potassium pump activity. Isolated heart cells were incubated in the following treatments: Rb-Krebs buffer without treatment (negative control), Ouabain+ Rb-Krebs buffer (positive control), …

Expression Of Fam171b Protein In Mouse Brain Tissue, Quan Tran

All Graduate Theses, Dissertations, and Other Capstone Projects

Polyglutamine (polyQ) diseases are inherited fatal neurodegenerative disorders caused by expansion of trinucleotide cytosine-adenine-guanine (CAG) repeats, encoding abnormally long glutamine tracts in respective disease proteins. Currently, there are nine polyQ diseases- including Huntington’s disease and a number of Spinocerebellar ataxias. Interestingly, expanded polyQ proteins are prone to aggregate, and this aggregation may underlie neurodegeneration. In this study, we investigate the expression and localization of FAM171B (a novel polyQ protein) in the brain. Western blotting reveals that FAM171B protein is indeed expressed in the developing and adult mouse brain. Furthermore, immunohistochemical analyses suggests widespread localization of FAM171B to many brain regions …

Zooplanktonic Community Dynamics Of The Minnesota River With An Ichthyoplankton Gear Comparison, Nathaniel Lederman

All Graduate Theses, Dissertations, and Other Capstone Projects

The Minnesota River, like many large rivers, has been functionally altered by human activities and climate change. The Minnesota Pollution Control Agency has designated 271 kilometers (50.3%) of the 539 kilometer Minnesota River as “biologically impaired.” However, assessing biological communities in large rivers is often difficult and limited to examination of upper trophic levels (e.g., piscivorous fishes). Few studies examined zooplanktonic communities, largely due to difficulties associated with sampling. Because of the need to improve assessment strategies for biological impairments in the Minnesota River, the zooplanktonic community, including crustaceous zooplankton, rotifers, macroinvertebrates, and ichthyoplankton was evaluated within an impaired and …

The Utilization Of Morphological And Genetic Diagnostic Techniques For The Description Of Trematode Species Collected From Waterbirds From Lake Winnibigoshish, Minnesota, Usa, Tyler Joseph Achatz

All Graduate Theses, Dissertations, and Other Capstone Projects

Historically, morphological techniques for species identification were the leading diagnostic methodology, however, the increased usage of genetic techniques has led to a decrease in reports of morphometrics. The decrease in morphological reports increases the chance of missing diagnostic morphometrics. The three studies described herein used morphological and genetic diagnostic methods to identify trematodes from five families in order to improve genetic and morphological information for trematode species identification.

The first study identified ten species of trematodes from intestines of waterbirds previously collected from Lake Winnibigoshish, Minnesota. Nine of the species were sequenced for 28S ribosomal DNA (rDNA). Two species were …

Cochlear Development And Auditory Function In The Absence Of Thyroid Hormone Transporters Mct8 And Oatp1c1, Richard Sinn

All Graduate Theses, Dissertations, and Other Capstone Projects

Thyroid hormone (TH) is essential for the development and maturation of the nervous system. The thyroid gland secretes an active form of TH, triiodothyronine, and a prohormone, thyroxine, into the blood. TH is charged, which prevents it from passively diffusing across cell membranes and thus requires cell membrane transporters to facilitate its movement into and out of cells. However, whether TH transporters are required for TH-mediated developmental events, including the auditory system, is largely unknown.

The purpose of the present study was to investigate two specific TH transporters, monocarboxylate transporter 8 (Mct8) and organic anion transporting polypeptide 1c1 (Oatp1c1), and …

Relationships Between Isolating Land Use And Amphibian Populations In Sub-Boreal Peatlands Of The Midwestern United States, Jeana Rose Albers

All Graduate Theses, Dissertations, and Other Capstone Projects

Successful conservation efforts of amphibians depend on the knowledge of habitat preferences because the biggest threat to amphibian populations is considered to be habitat loss. Sub-boreal peatlands in the Midwest may be a refuge for amphibian populations, but little is known about the flora and fauna of these peatlands. My study examined amphibian species richness and species diversity in 17 sub-boreal peatlands in Minnesota and Wisconsin in 2011 and 2012. I assessed the relationship between land use of lands adjacent to peatlands and use of those peatlands by amphibian populations at three spatial scales by examining landscapes surrounding peatlands in …

The Effects Of Harvest Regime, Irrigation, And Salinity On Stem Lignocellulosic Concentrations In Alfalfa (Medicago Sativa L.), Adam Harvey Warnke

All Graduate Theses, Dissertations, and Other Capstone Projects

Rapid consumption of crude oil reserves has made it necessary to find methods of processing a renewable and sustainable feedstock for conversion into ethanol. Lignocellulosic feedstocks are promising because they are typically environmentally friendly and can meet the high-yield potential necessary for ethanol production. Alfalfa (Medicago sativa L.) has promise as a feedstock for ethanol production because of its high biomass yields, perennial-habit, relationship with nitrogen-fixing bacteria, and other co-products. This study focused on the effects of harvest regime, irrigation, and salinity on stem lignocellulosic concentrations in alfalfa for ethanol production during the 2010 and 2011 growing seasons in …

Effect Of Low Maternal Aldosterone On Offspring Cardiovascular Development, Travis Dean Mckee

All Graduate Theses, Dissertations, and Other Capstone Projects

Maternal conditions are well known to affect fetal cardiovascular development. This study was designed to investigate in spontaneously hypertensive rats (SHR), the effects of maternal aldosterone suppression on the development of hypertension in the offspring. A sham procedure or a right adrenalectomy combined with cryodestruction of the zona glomerulosa of the left adrenal gland was performed on SHR females. The rats were mated and male offspring were implanted at 11-13 weeks of age with a PA-C40 remote monitoring device placed into the femoral artery to record cardiovascular parameters for 4 weeks. Systolic (mmHg), diastolic (mmHg), and mean blood pressure (mmHg), …

Influences Of Adjacent Land-Use On Avian Community Structure In Sub-Boreal Peatlands Of The Midwest, Lucas J. Wandrie

All Graduate Theses, Dissertations, and Other Capstone Projects

Surrounding land use influences avian community structure; moreover, anthropogenic manipulation of habitat can alter bird species richness and composition. In the first chapter of my thesis work, I conducted avian surveys at 20 sub-boreal peatlands in eastern Minnesota and western Wisconsin to detect correlations between land development and bird community structure. Peatlands are wetlands that accumulate decaying organic plant material (peat) and provide valuable and diverse habitats to a variety of flora and fauna. I measured urban and cropland development at three spatial scales (500 m, 1000 m, and 2500 m radii). Effects of development on avian communities also were …

Effect Of Process-Oriented Guided-Inquiry Learning On Non-Majors Biology Students' Understanding Of Biological Classification, Breann Marie Wozniak

All Graduate Theses, Dissertations, and Other Capstone Projects

The purpose of this study was to examine the effect of process-oriented guided-inquiry learning (POGIL) on non-majors college biology students' understanding of biological classification. This study addressed an area of science instruction, POGIL in the non-majors college biology laboratory, which has yet to be qualitatively and quantitatively researched. A concurrent triangulation mixed methods approach was used. Students' understanding of biological classification was measured in two areas: scores on pre and posttests (consisting of 11 multiple choice questions), and conceptions of classification as elicited in pre and post interviews and instructor reflections. Participants were Minnesota State University, Mankato students enrolled in …

Analysis Of The Genetic Structure Of Bithynia Tentaculata Snail Populations In Wisconsin And Minnesota, Sarah J. Whalen

All Graduate Theses, Dissertations, and Other Capstone Projects

In recent years, there have been tens of thousands of waterfowl mortalities in Wisconsin and Minnesota. An invasive species of snail, Bithynia tentaculata, is a host for the trematode parasites (Cyathocotyle bushiensis and Sphaeridiotrema globulus) that have caused these deaths. A microsatellite-enriched genomic library was detected using DNA from a B. tentaculata specimen from Lake Onalaska (Pool 7 of the Upper Mississippi River). Seven polymorphic microsatellite loci were used to genotype snails collected from Lake Butte des Morts, Shawano Lake, and Lake Onalaska in Wisconsin, as well as Lake Winnibigoshish in Minnesota. The genetic diversity of each population was measured …

Determining A Patient Recovery From A Total Knee Replacement Using Fuzzy Logic And Active Databases, Robert Azarbod

All Graduate Theses, Dissertations, and Other Capstone Projects

The purpose of the knowledge-based system is to predict the rehabilitation timeline of a patient in physical therapy for a total knee replacement. All patients have various attributes that contribute to their rehabilitation rate such as: weight, gender, smoking habit, medications, physical ability, or other medical problems. A combination of any one or several of these attributes will affect the recovery process. The proposed FRTP (Fuzzy Rehabilitation Timeline Predictor) is a fuzzy data mining model that can predict the recovery length of a patient in physical therapy for a total knee replacement and provide feedback to experts for revision of …

Class Discovery And Prediction Of Tumor With Microarray Data, Bo Liu

All Graduate Theses, Dissertations, and Other Capstone Projects

Current microarray technology is able take a single tissue sample to construct an Affymetrix oglionucleotide array containing (estimated) expression levels of thousands of different genes for that tissue. The objective is to develop a more systematic approach to cancer classification based on Affymetrix oglionucleotide microarrays. For this purpose, I studied published colon cancer microarray data. Colon cancer, with 655,000 deaths worldwide per year, has become the fourth most common form of cancer in the United States and the third leading cause of cancer - related death in the Western world. This research has been focuses in two areas: class discovery, …

Floristic And Environmental Characteristics Of Sub-Boreal Peatlands Of Minnesota And Western Wisconsin, Kevin Douglas Clement

All Graduate Theses, Dissertations, and Other Capstone Projects

Peatland communities in the sub-boreal region of Minnesota and western Wisconsin were characterized by floristic composition, structure and environmental characteristics to determine the natural variation among peatland communities in the region and their shared associations with the boreal and temperate peatlands of North America. Floristic classification revealed five, distinct peatland community types, identified as Sphagnum Bogs, Herbaceous Fens, Forested Fens, Rich/Calcareous Fens and Phalaris anrundinacea dominated peatlands. Differences among sub-boreal peatlands largely reflected changes in the significant indicator and dominant plant species identified, which were found to exhibit high fidelities to a particular peatland community type. Floristic variations among sub-boreal …