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Impact Of Mtr4 Structural Domains On Its Enzymatic Activities, In The Regulation Of Nuclear Rna Turnover, Li Yan

Dissertations (1934 -)

RNA processing and turnover plays an important role in RNA maturation, metabolism and quality control, which contribute to gene regulation and cell health. The TRAMP complex, composed of an RNA binding protein Air2p, a poly-A polymerase Trf4p, and a RNA helicase Mtr4p, assist nuclear exosome-dependent RNA processing and degradation in Saccharomyces cerevisiae, like tRNAiMet turnover, 5'ETS degradation and rRNA processing. Mtr4p structure reveals a novel protruding arch domain, which contains the recognizable KOW domain and a stalk domain. Except for the two conserved RecA-like helicase domains, the helicase core contains two other structural domains, a winged helix that connects arch …