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Environmental, Genotypic, And Ploidy Effects On Endopolyploidization Within A Genotype Of Solanum Phureja And Its Derivatives, Henry R. Owen, R. E. Veilleux, D. Levy, D. L. Ochs
Environmental, Genotypic, And Ploidy Effects On Endopolyploidization Within A Genotype Of Solanum Phureja And Its Derivatives, Henry R. Owen, R. E. Veilleux, D. Levy, D. L. Ochs
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Flow-cytometric analyses of DNA content were performed on chopped in vivo, in vitro, and protoplast-derived in vitro nuclei of Solanum phureja. An anther-derived monoploid genotype and a diploid and tetraploid clone, derived from callus culture of the monoploid genotype, were used to characterize the influence of in vivo and in vitro environment and explant ploidy level on the extent of endopolyploidization. In addition, protoplast-derived nuclei, from nine anther-derived monoploid genotypes, were examined for genotypic influences on endopolyploidization. DNA distributions of the anther-derived monoploid and callus-derived clones in vivo contained peaks corresponding to 1C, 2C, and 4C DNA levels. By comparison, …