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Full-Text Articles in Biology
Meiosis In Budding Yeast, G. Valentin Borner, Andreas Hochwagen, Amy J. Macqueen
Meiosis In Budding Yeast, G. Valentin Borner, Andreas Hochwagen, Amy J. Macqueen
Biological, Geological, and Environmental Faculty Publications
Meiosis is a specialized cell division program that is essential for sexual reproduction. The two meiotic divisions reduce chromosome number by half, typically generating haploid genomes that are packaged into gametes. To achieve this ploidy reduction, meiosis relies on highly unusual chromosomal processes including the pairing of homologous chromosomes, assembly of the synaptonemal complex, programmed formation of DNA breaks followed by their processing into crossovers, and the segregation of homologous chromosomes during the first meiotic division. These processes are embedded in a carefully orchestrated cell differentiation program with multiple interdependencies between DNA metabolism, chromosome morphogenesis, and waves of gene expression …
Identification Of New Genes Involved In Meiosis By A Genetic Screen, Sneharthi Banerjee
Identification Of New Genes Involved In Meiosis By A Genetic Screen, Sneharthi Banerjee
ETD Archive
Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins have defects in recombination, SC formation and nuclear division progression. Meiotic cell cycle progression in zmm mutants is modulated by temperature. This conditional behavior is different at high and low temperatures. In my work so far, I have tried to identify new zmm-like genes involved in meiosis. To that end, I have carried out a genome-wide screen in the budding yeast S. cerevisiae. I have identified sporulation temperature sensitive zmm-like truncation mutants …
Responses To Low Double-Strand Break Levels In Budding Yeast Meiosis, Scott A. Gaskell
Responses To Low Double-Strand Break Levels In Budding Yeast Meiosis, Scott A. Gaskell
ETD Archive
During meiosis, one round of DNA replication is followed by two rounds of chromosome segregation, producing four haploid gametes from each diploid precursor cell. Self-inflicted DNA double-strand breaks (DSBs) occur in prophase of meiosis I. A subset of DSBs are repaired using the homologous chromosome as template for homologous recombination, generating crossovers/chiasmata. When processing of DSBs is defective, the recombination checkpoint delays onset of the first meiotic cell division. Here, I have investigated mechanisms by which the budding yeast S. cerevisiae responds to low levels of initiating DSBs. A novel checkpoint is identified that is specifically triggered by low DSB …