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Articles 1 - 7 of 7
Full-Text Articles in Biology
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes fatal human African trypanosomiasis and evades the host immune response by regularly switching its major surface antigen, VSG, which is expressed exclusively from subtelomeric loci. Telomere length and telomere proteins play important roles in regulating VSG silencing and switching. T. brucei telomerase plays a key role in maintaining telomere length, and T. brucei telomeres terminate in a single-stranded 3′ G-rich overhang. Understanding the detailed structure of the telomere G-overhang and its maintenance will contribute greatly to better understanding telomere maintenance mechanisms. Using an optimized adaptor ligation assay, we found that most T. brucei telomere G-overhangs end in …
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes human African trypanosomiasis and regularly switches its major surface antigen, VSG, thereby evading the host's immune response. VSGs are monoallelically expressed from subtelomeric expression sites (ESs), and VSG switching exploits subtelomere plasticity. However, subtelomere integrity is essential for T. brucei viability. The telomeric transcript, TERRA, was detected in T. brucei previously. We now show that the active ES-adjacent telomere is transcribed. We find that TbRAP1, a telomere protein essential for VSG silencing, suppresses VSG gene conversionmediated switching. Importantly, TbRAP1 depletion increases the TERRA level, which appears to result from longer read-through into the telomere downstream of the …
Cryptochromes Regulate Igf-1 Production And Signaling Through Control Of Jak2-Dependent Stat5b Phosphorylation, Amol Santosh Charudhari
Cryptochromes Regulate Igf-1 Production And Signaling Through Control Of Jak2-Dependent Stat5b Phosphorylation, Amol Santosh Charudhari
ETD Archive
Insulin-like growth factor (IGF) signaling plays an important role in cell growth and proliferation and is implicated in regulation of cancer, metabolism, and aging. Here we report that IGF-1 level in blood and IGF-1 signaling demonstrates circadian rhythms. Circadian control occurs through cryptochromes (CRYs)—transcriptional repressors and components of the circadian clock. IGF-1 rhythms are disrupted in Cry-deficient mice, and IGF-1 level is reduced by 80% in these mice, which leads to reduced IGF signaling. In agreement, Cry-deficient mice have reduced body (~30% reduction) and organ size. Down-regulation of IGF-1 upon Cry deficiency correlates with reduced Igf-1 mRNA expression in the …
Restoration Of Black Oak (Quercus Velutina) Sand Barrens Via Three Different Habitat Management Approaches, David J. Kriska
Restoration Of Black Oak (Quercus Velutina) Sand Barrens Via Three Different Habitat Management Approaches, David J. Kriska
ETD Archive
Disturbance regimes, i.e. frequent fires, historically maintained oak barrens until European settlement patterns, and eventually, Smoky the Bear and the fire suppression campaign of the U.S. Forest Service snuffed out the periodic flames. In the absence of a disturbance regime, ground layer floral composition at many historical oak sand barrens will change predominantly because of a buildup of leaf litter and shading of the soils. Termed mesophication, this process of ecological succession will drive Black Oak Sand Barrens to an alternate steady state. A survey conducted on Singer Lake Bog in Green, Ohio, demonstrated that succession shifted the community to …
Age And Sex-Specific Effect Of Caloric Restriction On Circadian Clock And Longevity-Associated Gene Expression, Arten Andreyevich Astafev
Age And Sex-Specific Effect Of Caloric Restriction On Circadian Clock And Longevity-Associated Gene Expression, Arten Andreyevich Astafev
ETD Archive
The rhythms in the expression of circadian clock genes are affected by calorie restriction (CR), a dietary paradigm known to increase lifespan. In our current study, we show that circadian rhythms are influenced by sex and the effects of CR are different between males and females. In particular, we found a group of clock genes which showed a sex-dependent difference in expression, as well as in response to CR (Rev-Erb α, Ror γ and both Cryptochromes: Cry1 and Cry2 genes). Two clock genes showed no difference in expression but their response to CR showed sexual dimorphism (Ror α and Rev-Erb …
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Biological, Geological, and Environmental Faculty Publications
The primary objective of a new survey of the Cuyahoga River was to assess species richness and population abundance of various mussel species in the family Unionidae throughout the Middle Cuyahoga River and upper parts of the Lower Cuyahoga River. Historically, few records existed for this river between Lake Rockwell and the Cuyahoga Valley National Park, a region in which four dams have been removed in the past 12 years. Timed visual surveys were conducted during low flow conditions and by using tactile techniques in deeper water. Throughout the survey, only 37 live animals were located across the 18 Middle …
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Biological, Geological, and Environmental Faculty Publications
In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying “doughnut-shaped” vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins. Giemsa staining and immunofluorescent microscopy identified different developmental stages of Colpodella trophozoites, …