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California Polytechnic State University, San Luis Obispo

Biological Sciences

2003

DNA extraction; DNA purification; PCR inhibition

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Removal Of Pcr Inhibitors From Soil Dna By Chemical Flocculation, Michael D. Braid, Laura M. Daniels, Christopher L. Kitts Mar 2003

Removal Of Pcr Inhibitors From Soil Dna By Chemical Flocculation, Michael D. Braid, Laura M. Daniels, Christopher L. Kitts

Biological Sciences

Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH4(SO4)2 during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.

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