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Characterization Of The Life Cycle And Heteromeric Nature Of The Macronucleus Of The Ciliate Chilodonella Uncinata Using Fuorescence Microscopy, Laure Bellec, Xyrus X. Maurer-Alcala, Laura A. Katz
Characterization Of The Life Cycle And Heteromeric Nature Of The Macronucleus Of The Ciliate Chilodonella Uncinata Using Fuorescence Microscopy, Laure Bellec, Xyrus X. Maurer-Alcala, Laura A. Katz
Biological Sciences: Faculty Publications
Only a limited number of studies exist on the life cycles of nonmodel ciliates such as Chilodonella uncinata (Cl: Phyllopharyngea). The handful of papers on this taxon indicate the presence of a heteromeric macronucleus, marked by separate DNA-rich and DNA-poor regions. Here, we study the life cycle of C. uncinata using confocal laser scanning microscopy with 4′,6-diamidino-2- phenylindole staining, which allows us to differentiate nuclear dynamics of the micronucleus and the macronucleus during life-cycle stages. We photo-documented various stages and confirmed aspects of the development of the new macronucleus previously characterized by electron microscopy. We further reveal the heteromeric structure …