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Maldi-Tof Ms As A Supportive Tool For The Evaluation Of Bacterial Diversity In Soils From Africa And The Americas, Adrian Douglas Allen, Maria Velez-Quinones, Broderick Eribo, Vernon Morris
Maldi-Tof Ms As A Supportive Tool For The Evaluation Of Bacterial Diversity In Soils From Africa And The Americas, Adrian Douglas Allen, Maria Velez-Quinones, Broderick Eribo, Vernon Morris
Broderick Eribo
Identification and characterization of viable-culturable bacteria (VCB) associated with soils from Africa and the Americas is significant for environmental and battlefield security. Such analyses are scarce and their evaluation using traditional microbiological methods do not fully elucidate the structure and chemotaxonomic characteristics of the microbial community. In this study, matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) spectrometry in addition to 16S rRNA sequencing, and diversity indices were employed to characterize VCB and their associated biomarkers. Nineteen genera were identified across all sample locations, but only four (Bacillus, Brevibacillus, Paenibacillus and Terribacillus) confirmed by ClustalW2 as being …