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Full-Text Articles in Biology
The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky
The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky
Biology Faculty Works
Background: High temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.Results: The promoter of Hsp17.6 was used to build a Hsp17.6(p):GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p: GFP response dynamics. Hsp17.6(p):GFP signal increased with temperature increases from …
The Effect Of Temperature Extremes On Cardiorespiratory Function In The Grass Shrimp Palaemonetes Pugio: Oxygen Limited Thermal Tolerance, Teresa Mika
UNLV Theses, Dissertations, Professional Papers, and Capstones
Aquatic poikilothermic animals must either cope with or compensate for the mismatch in oxygen supply and demand present at high temperatures. Oxygen limited thermal tolerance explains how aerobic scope is limited by insufficient oxygen supply and sets the performance window in animals. This work explores the effects of temperature on the different components of the oxygen delivery system, in both normoxic and hyperoxic conditions to determine if supplying more oxygen to the system mitigates the effects of temperature effects on the cardiorespiratory system and extends oxygen limited thermal tolerance to higher temperatures. The effect of temperature and oxygen condition was …