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Assessing Student Comprehension In Introductory Biology: A Comparison Of Free-Response And Multiple-True/False Exam Formats, Macy A. Potts, Brian Couch, Joanna K. Hubbard
Assessing Student Comprehension In Introductory Biology: A Comparison Of Free-Response And Multiple-True/False Exam Formats, Macy A. Potts, Brian Couch, Joanna K. Hubbard
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Question format is an important consideration when designing instruments to gauge student comprehension. In many lecture-style courses, instructors must decide how to effectively test a large group of students. Two common types of question formats used for introductory biology exams are free-response (FR) and multiple-true/false (MTF). FR questions include a question prompt which students respond to with essay-style answers. Conversely, MTF questions include an opening question stem with accompanying statements to be marked as either true or false. The goal of this study was to determine the advantages and disadvantages of FR and MTF question formats for assessing student comprehension …
Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Articles
The goal of this protocol is to allow for the rapid verification of bioinformatically identified terminators. Further, the plasmid (pGR-Blue) is designed specifically for this protocol and allows for the quantification of terminator efficiency. As a proof of concept, six terminators were bioinformatically identified in the mycobacteriophage Bernal13. Once identified, terminators were then made as oligonucleotides with the appropriate sticky ends and annealed together. Using Golden Gate Assembly (GGA), terminators were then cloned into pGR-Blue. Under visible light, false positive colonies appear blue and positively transformed colonies are white/yellow. After induction of an arabinose inducible promoter (pBad) with arabinose, colony …