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A Kinesin-Like Calmodulin-Binding Protein In Chlamydomonas: Evidence For A Role In Cell Division And Flagellar Functions, Erin E. Dymek, Daniel Goduti, Tal Kramer, Elizabeth F. Smith
A Kinesin-Like Calmodulin-Binding Protein In Chlamydomonas: Evidence For A Role In Cell Division And Flagellar Functions, Erin E. Dymek, Daniel Goduti, Tal Kramer, Elizabeth F. Smith
Dartmouth Scholarship
Kinesin-like calmodulin-binding protein, KCBP, is a novel member of the C-kinesin superfamily first discovered in flowering plants. This minus-end-directed kinesin exhibits Ca(2+)-calmodulin-sensitive motor activity in vitro and has been implicated in trichome morphogenesis and cell division. A homologue of KCBP is also found in the unicellular, biflagellate green alga Chlamydomonas reinhardtii (CrKCBP). Unlike plant cells, Chlamydomonas cells do not form trichomes and do not assemble a phragmoplast before cell division. To test whether CrKCBP is involved in additional microtubule-based processes not observed in plants, we generated antibodies against the putative calmodulin-binding domain and used these antibodies in biochemical and localization …