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Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Theses and Dissertations - UTB/UTPA
In this study the functional characterization and overexpression of Metal Tolerance Protein (MTP) cDNA were evaluated using a transgenic yeast (Saccharomyces cerevisiae) model system as a preliminary means for the characterization of metal tolerance conferred by putative Group III MTP genes from Arabidopsis thaliana. Metal disc assays for nickel, zinc, cobalt and cadmium treatments were performed to measure the tolerance imparted to yeast by the MTP cDNAs. Statistical analysis from zinc and cadmium assays showed AtMTP1 imparted the highest zinc tolerance in yeast, while AtMTPB1 imparted the highest cadmium and cobalt tolerance. However, AtMTPA2 and AtMTPB produced a decrease in …
Use Of Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction (Rapd-Pcr) To Distinguish North American Populations Of Acroptilon Repens (L.) Dc, Raul A. Ruiz
Theses and Dissertations - UTB/UTPA
Sixty U.S. populations of Acroptilon repens collected in 1998 were analyzed using RAPD-PCR. Each population was represented by five specimens gathered from each location. A total of 301 specimens were examined using two Operon Technologies, RAPD-PCR primers (A 10 and C04). The DNA amplification products produced through the PCR process were electrophoretically separated to produce DNA fragments ranging from 100 to 2000 base-pairs in length. The 60 U.S. populations sampled, produce ten RAPD banding pattern types using A 10 primer and seventeen pattern types with C04. Genetic variation within and among the populations was low, however, one dendogram did show …