Biodiversity Commons^™

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson

Journal of the South Carolina Academy of Science

Bacteriophages are used as indicators of pathogenic bacteria in drinking, and wastewaters. They also show potential in limiting aquatic bacterial populations through their lytic properties. The effect of different water characteristics (salinity, pH, dissolved oxygen, and temperature) on the sensitivity of the PCR identification of virus particles were analyzed to determine at what levels bacteriophage can be detected in environmental samples. Results from this preliminary study indicate that a PCR bacteriophage detection technique has potential as a relatively efficient and economical indicator of coliform contamination in multiple aquatic environments. While further evaluation is needed, the protocol appears to function in …

Variable Δ15n Diet-Tissue Discrimination Factors Among Sharks: Implications For Trophic Position, Diet And Food Web Models, Jill A. Olin, Nigel E. Hussey, Alice Grgicak-Mannion, Mark W. Fritts, Sabine T. Wintner, Aaron T. Fisk

Great Lakes Institute for Environmental Research Publications

The application of stable isotopes to characterize the complexities of a species foraging behavior and trophic relationships is dependent on assumptions of δ15N diet-tissue discrimination factors (∆15N). As ∆15N values have been experimentally shown to vary amongst consumers, tissues and diet composition, resolving appropriate speciesspecific ∆15N values can be complex. Given the logistical and ethical challenges of controlled feeding experiments for determining ∆ 15N values for large and/or endangered species, our objective was to conduct an assessment of a range of reported ∆ 15N values that can hypothetically serve as surrogates for describing the predator-prey relationships of four shark species …

Diatom Assemblages Promote Ice Formation In Large Lakes, N. A. D'Souza, Y. Kawarasaki, J. D. Gantz, R. E. Lee, B. F.N. Beall, Y. M. Shtarkman, Z. A. Koçer, S. O. Rogers, H. Wildschutte, G. S. Bullerjahn, R. M.L. Mckay

Great Lakes Institute for Environmental Research Publications

We present evidence for the directed formation of ice by planktonic communities dominated by filamentous diatoms sampled from the ice-covered Laurentian Great Lakes. We hypothesize that ice formation promotes attachment of these non-motile phytoplankton to overlying ice, thereby maintaining a favorable position for the diatoms in the photic zone. However, it is unclear whether the diatoms themselves are responsible for ice nucleation. Scanning electron microscopy revealed associations of bacterial epiphytes with the dominant diatoms of the phytoplankton assemblage, and bacteria isolated from the phytoplankton showed elevated temperatures of crystallization (T c) as high as -3 °C. Ice nucleation-active bacteria were …

Aerobic Degradation Of Α-, Β-, Γ-Hexachlorocyclohexane By Narragansett Bay Bacterioplankton, Ian M. Rambo

Senior Honors Projects

Hexachlorocyclohexanes (HCHs) are a family of chlorinated organic compounds that were previously used as agricultural insecticides. HCHs are recognized as persistent organic pollutants due to their toxicity, recalcitrant properties, and tendency to bioaccumulate in food webs. Although HCH was first synthesized in 1825, its use was not widespread until the discovery of the insecticidal activity of the γ-HCH isomer in 1942. γ-HCH and its toxic waste isomers α-HCH and β-HCH were banned from production and use by the United Nations in 2009, yet these chemicals still present environmental problems due to their persistence in soils and surface waters. HCHs continue …

Effects Of Synthetic Histone Deacetylase Inhibitors On 3t3l1 Preadipocyte Adipogenic Differentiation Potential, Vikramjeet Singh

Honors Theses

No abstract provided.

Acute Toxicity Of Copper Sulfate And Potassium Dichromate On Stygobiont Proasellus: General Aspects Of Groundwater Ecotoxicology And Future Perspectives, Ana Reboleira, Nelson Abrantes, Pedro Oromí, Fernando Gonçalves

Ana Sofia P.S. Reboleira

Karst systems harbor large groundwater resources for human consumption and represent an important habitat for rare and unprotected specialized animals, the so-called stygofauna. Due to the highly adapted features towards underground life, together with the geographic isolation provided by the subterranean aquifers, groundwater-dwelling animals may lose the ability to face sudden changes on their ecosystems, and therefore the risk of extinction is remarkably high. A little is known about their sensitiveness, especially linked to contamination pressure in urbanized karst areas. Understanding the impact of contaminants on stygofauna is important for setting groundwater environmental quality and management of karst systems. We …

Rates And Controls Of Nitrification In A Large Oligotrophic Lake, Gaston E. Small, George S. Bullerjahn, Robert W. Sterner, Benjamin F.N. Beall, Sandra Brovold, Jacques C. Finlay, Robert M.L. Mckay, Maitreyee Mukherjee

Great Lakes Institute for Environmental Research Publications

Recent discoveries have altered prevailing paradigms concerning the conditions under which nitrification takes place and the organisms responsible for nitrification in aquatic ecosystems. In Lake Superior, nitrate (NO-3) concentrations have increased fivefold in the past century. Although previous evidence indicated that most NO-3 is generated by nitrification within the lake, important questions remain concerning the magnitude and controls of nitrification, and which microbial groups are primarily responsible for this process. We measured water-column nitrification rates in the western basin of Lake Superior during five research cruises from November 2009 to March 2011. Using in situ bottle incubations at 10 depths, …

Agricultural Geo-Engineering; Past, Present & Future, Erich J. Knight Mr.

Erich J Knight Mr.

Historic hall marks of GHG emissions are reviewed, providing repeated demonstration of anthropogenic land use changes on climate forcing.

New Astrophysical and Paleoclimate concordance with extinction events demonstrating climate adaptation by prehistoric man.

A review of new research concerning Soil Carbon, Carboniferous Aerosols, extent of Pyrolytic-Carbon fraction in soil and the first survey of the extensive deep soil carbon sink.

How thermal conversion technologies can integrate and optimize the recycling of valuable nutrients while providing energy and building soil carbon.

New discoveries from the Advanced Spectrometry & Meta-Genomics studies in soil microbiology which demonstrate unaccounted for ecological services provided by …

Bacteriostatic Effects Of Sucralose On Environmental Bacteria, Arthur Phillip Omran Jr.

UNF Graduate Theses and Dissertations

Sucralose is a zero calorie sweetener developed and manufactured by Tate and Lyle Sweetener Company in the 1980’s. They sell the sweetener compounded with maltodextrin and dextrose under the brand name Splenda®. Sucralose was developed as a low cost artificial sweetener that is non-metabolizable in humans and can withstand changes in pH and temperature. It is not degraded by the waste water treatment process. Since the molecule can withstand heat, acidification and microbial degradation it is accumulating in the environment, and has been found in waste water, estuaries, rivers and the Gulf Stream. The highest concentration of environmental sucralose detected …