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Biodiversity Commons

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Environmental Microbiology and Microbial Ecology

Journal

Bacteria

Publication Year

Articles 1 - 2 of 2

Full-Text Articles in Biodiversity

Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann Jun 2019

Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann

International Journal of Speleology

Tunnels in a warm, humid area of the 1478 m level of the Sanford Underground Research Facility (SURF), located in a former gold mine in South Dakota, USA, host irregular, thin whitish, iridescent biofilms, which appear superficially similar to ‘cave silver’ biofilms described from limestone and lava tube caves, despite the higher rock temperature (32°C) and differing rock surface (phyllite) present at SURF. In this study, we investigated the diversity of cultivable bacteria constituting the cave silver by using several media: CN agar, CN gellan gum and 0.1X R2A agar. The highest colony count (CFU/g of sample) was observed on …


Diversity Of Cultured Bacteria From The Perennial Ice Block Of Scarisoara Ice Cave, Romania, Corina Iţcuş, Mădălina-Denisa Pascu, Traian Brad, Aurel Perşoiu, Cristina Purcarea Jan 2016

Diversity Of Cultured Bacteria From The Perennial Ice Block Of Scarisoara Ice Cave, Romania, Corina Iţcuş, Mădălina-Denisa Pascu, Traian Brad, Aurel Perşoiu, Cristina Purcarea

International Journal of Speleology

Cave ice ecosystems represent a poorly investigated glacial environment. Diversity of cave ice bacteria and their distribution in perennial ice deposits of this underground glacial habitat could constitute a proxy for microbial response to climatic and environmental changes. Scarisoara Ice Cave (Romania) hosts one of the oldest and largest cave ice blocks worldwide. Here we report on cultured microbial diversity of recent, 400, and 900 years-old perennial ice from this cave, representing the first characterization of a chronological distribution of cave-ice bacteria. Total cell density measured by SYBR Green I epifluorescence microscopy varied in the 2.4 x 104 – …