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Protocol For Rapid Assessment Of The Efficacy Of Novel Wnt Inhibitors Using Zebrafish Models, Meghan G. Haney, Mary Wimsett, Chunming Liu, Jessica S. Blackburn

Molecular and Cellular Biochemistry Faculty Publications

Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing.

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