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Articles 1 - 13 of 13
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski
Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski
Amy Sprenkle
Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David S. Peabody
Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David S. Peabody
Marc Spingola
Thermodynamics Of Oligoarginines Binding To Rna And Dna., David Mascotti, Timothy Lohman
Thermodynamics Of Oligoarginines Binding To Rna And Dna., David Mascotti, Timothy Lohman
David P. Mascotti
These results suggest that hydrogen bonding of arginine to the phosphate backbone of the nucleic acids contributes to the increased stability of these complexes.
Ac-Mode Atomic Force Microscope Imaging In Air And Solutions With A Thermally Driven Bimetallic Cantilever Probe, Andrew C. Hillier, Allen J. Bard
Ac-Mode Atomic Force Microscope Imaging In Air And Solutions With A Thermally Driven Bimetallic Cantilever Probe, Andrew C. Hillier, Allen J. Bard
Andrew C. Hillier
An ac imaging mode for atomic force microscopy ~AFM! has been developed that employs a thermally driven bimetallic cantilever to sense surface topography. Oscillations are induced in a composite cantilever, comprising a Si3N4 layer and Au overcoat, by local heating with a resistive wire heater placed in close proximity to the cantilever. Cantilever bending occurs upon heating due to the difference in thermal expansion coefficients of the Si3N4 and Au layers. The magnitude of this bending is a function of the heat input, the cantilever geometry, the frequency of the excitation, and the thermal properties of the surrounding medium. A …
Rna Base-Amino Acid Interaction Strengths Derived From Structures And Sequences, Brooke Lustig, S Arora, R L. Jernigan
Rna Base-Amino Acid Interaction Strengths Derived From Structures And Sequences, Brooke Lustig, S Arora, R L. Jernigan
Brooke S. Lustig
We investigate RNA base-amino acid interactions by counting their contacts in structures and their implicit contacts in various functional sequences where the structures can be assumed to be preserved. These frequencies are cast into equations to extract relative interaction energetics. Previously we used this approach in considering the major groove interactions of DNA, and here we apply it to the more diverse interactions observed in RNA. Structures considered are the three different tRNA synthetase complexes, the U1A spliceosomal protein with an RNA hairpin and the BIV TARTat complex. We use binding data for the base frequencies for the seryl, aspartyl …
Complexes Between Nascent Polypeptides And Their Molecular Chaperones In The Cytosol Of Mammalian Cells, Daryl K. Eggers, W. J. Welch, W. J. Hansen
Complexes Between Nascent Polypeptides And Their Molecular Chaperones In The Cytosol Of Mammalian Cells, Daryl K. Eggers, W. J. Welch, W. J. Hansen
Daryl K. Eggers
Folding of newly synthesized proteins in vivo is believed to be facilitated by the cooperative interaction of a defined group of proteins known as molecular chaperones. We investigated the direct interaction of chaperones with nascent polypeptides in the cytosol of mammalian cells by multiple methods. A new approach using a polyclonal antibody to puromycin allowed us to tag and capture a population of truncated nascent polypeptides with no bias as to the identity of the bound chaperones. In addition, antibodies that recognize the cytosolic chaperones hsp70, CCT (TRiC), hsp40, p48 (Hip), and hsp90 were compared on the basis of their …
The Role Of Molecular Recognition In Activation And Regulation In The Growth Hormone-Prolactin Family Of Hormones And Receptors, Anthony Kossiakoff, Celia Schiffer, Abraham De Vos
The Role Of Molecular Recognition In Activation And Regulation In The Growth Hormone-Prolactin Family Of Hormones And Receptors, Anthony Kossiakoff, Celia Schiffer, Abraham De Vos
Celia A. Schiffer
In: Rick L. Ornstein, Biomacromolecules--from 3-D to applications: Thirty-fourth Hanford Symposium on Health and the Environment, October 23-26, 1995, Pasco, Washington, U.S.A. Battelle Press, 1997, p. 123-131. ISBN 1574770195, 9781574770193.
Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank
Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank
Fabienne Paumet
No abstract provided.
Specificity And Cellular Mechanism Of Lysophosphatidylcholine Stimulation Of Vascular Smooth Muscle Cell Growth., Yuh-Cherng Chai, David Binion, Guy Chisolm
Specificity And Cellular Mechanism Of Lysophosphatidylcholine Stimulation Of Vascular Smooth Muscle Cell Growth., Yuh-Cherng Chai, David Binion, Guy Chisolm
Yuh-Cherng Chai
No abstract provided.
Time-Averaging Crystallographic Refinement, Celia Schiffer
Time-Averaging Crystallographic Refinement, Celia Schiffer
Celia A. Schiffer
In: Wilfred F. van Gunsteren and Paul K. Weiner, Computer Simulation of Biomolecular Systems: Theoretical and Experimental Applications (Volume 3 of Computer Simulation of Biomolecular Systems), Springer, 1997, p. 265-269. ISBN 9072199251, 9789072199256.
Full text of chapter is available through Google Book Search limited preview: Google Book Search description
The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock
The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock
Roberta Pollock
The transcription factor Pax-5 is expressed during the early stages of B-cell differentiation and influences the expression of several B-cell-specific genes. In addition to the existing isoform (Pax-5, which we have named Pax-5a), we have isolated three new isoforms, Pax-5b, Pax-5d, and Pax-5e, from murine spleen and B-lymphoid cell lines using library screenings and polymerase chain reaction amplification. Isoforms Pax-5b and Pax-5e have spliced out their second exon, resulting in proteins with only a partial DNA-binding domain. Isoforms Pax-5d and Pax-5e have deleted the 3′-region, which encodes the transactivating domain, and replaced it with a novel sequence. The existence of …
Direct Measurement Of Diffuse Double-Layer Forces At The Semiconductor/Electrolyte Interface Using An Atomic Force Microscope, Kai Hu, Fu-Ren F. Fan, Allen J. Bard, Andrew C. Hillier
Direct Measurement Of Diffuse Double-Layer Forces At The Semiconductor/Electrolyte Interface Using An Atomic Force Microscope, Kai Hu, Fu-Ren F. Fan, Allen J. Bard, Andrew C. Hillier
Andrew C. Hillier
The forces between a silica probe and an n-type TiO2 single-crystal electrode were measured using an atomic force microscope in an aqueous electrolyte solution. These interactions were a strong function of the solution pH, the presence of specifically adsorbed anions, and the TiO2 electrode potential. For a series of pH values, a strong electrostatic repulsion was seen at high pH and decreased as the pH was reduced. At pH values below 5.5, the interaction became attractive. A series of force measurements between SiO2 and n-type TiO2 showed a repulsive interaction when TiO2 was held at negative electrode potentials, which transformed …
Genetic Analysis Of Chlorophyll Biosynthesis, David W. Bollivar, Jon Y. Suzuki, Carl E. Bauer
Genetic Analysis Of Chlorophyll Biosynthesis, David W. Bollivar, Jon Y. Suzuki, Carl E. Bauer
David Bollivar
During this decade, there have been major advancements in the understanding of genetic loci involved in synthesis of the family of Mg-tetrapyrroles known as chlorophylls and bacteriochlorophylls. Molecular genetic analysis of Mg-tetrapyrrole biosynthesis was initiated by the performance of detailed sequence and mutational analysis of the photosynthesis gene cluster from Rhodobacter capsulatus. These studies provided the first detailed understanding of genes involved in bacteriochlorophyll a biosynthesis. In the short time since these studies were initiated, most of the chlorophyll biosynthesis genes have been identified by virtue of their ability to complement bacteriochlorophyll a biosynthesis mutants as well as by sequence …