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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Mechanistic Study Of Heme Protein-Mediated Nitric Oxide Dioxygenation Using Photolytically Produced Nitric Oxide, Karl Joseph Koebke
Mechanistic Study Of Heme Protein-Mediated Nitric Oxide Dioxygenation Using Photolytically Produced Nitric Oxide, Karl Joseph Koebke
Theses and Dissertations
The previously reported NO precursor [Mn(PaPy2Q)(NO)]ClO4 (1), where (PaPy2QH) is N,N-bis(2-pyridylmethyl)-amine-N-ethyl-2-quinoline-2-carboxamide, was synthesized and proven capable of producing as much as 180 µM NO when irradiated by a single 3 mJ 500nm laser pulse, in a 0.15 cm path cell, without the need for additional sacrificial reductants or oxidants. Species 1 was first used to study the reaction of nitric oxide with oxy-myoglobin (oxyMb) to form ferric myoglobin (metMb) and nitrate. This reaction had long been assumed to proceed via the same iron-bound peroxynitrite intermediate (metMb(OONO)) as the metMb-catalyzed isomerization of peroxynitrite to nitrate. Recent research showed that the metMb-catalyzed …
Molecular Identification Of Mycobacterium Tuberculosis In The Milwaukee County Institution Grounds Cemetery, Helen Marie Werner
Molecular Identification Of Mycobacterium Tuberculosis In The Milwaukee County Institution Grounds Cemetery, Helen Marie Werner
Theses and Dissertations
The possibility of identifying Mycobacterium tuberculosis in skeletal remains has been a debated topic for many years. This study utilizes the remains from the 1991 and 1992 excavations of the Milwaukee County Institution Grounds Cemetery, a collection of human skeletons ranging from 1882 to 1925, of various ages and sexes, to address that possibility. To test the utility of previously used methods of osteological identification of tuberculosis, the collection has been analyzed for the IS6110 repetitive element marker using molecular biological techniques, such as Polymerase Chain Reaction (PCR). Eighty-six skeletons from the collection have been analyzed, with nine of them …