Open Access. Powered by Scholars. Published by Universities.®
Biochemistry, Biophysics, and Structural Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Large Intracellular Loop Of Hzip4 Is An Intrinsically Disordered Zinc Binding Domain, Robert Dempski, Elizabeth Bafaro, Sagar Antala, Tuong-Vi Nguyen, Stephen Dzul, Brian Doyon, Timothy Stemmler
The Large Intracellular Loop Of Hzip4 Is An Intrinsically Disordered Zinc Binding Domain, Robert Dempski, Elizabeth Bafaro, Sagar Antala, Tuong-Vi Nguyen, Stephen Dzul, Brian Doyon, Timothy Stemmler
Robert E. Dempski
The human (h) ZIP4 transporter is a plasma membrane protein which functions to increase the cytosolic concentration of zinc. hZIP4 transports zinc into intestinal cells and therefore has a central role in the absorption of dietary zinc. hZIP4 has eight transmembrane domains and encodes a large intracellular loop between transmembrane domains III and IV, M3M4. Previously, it has been postulated that this domain regulates hZIP4 levels in the plasma membrane in a zinc-dependent manner. The objective of this research was to examine the zinc binding properties of the large intracellular loop of hZIP4. Therefore, we have recombinantly expressed and purified …
The Role Of Histidine Residues In The Specificity Of The Human Zinc Transporter Hzip4, Robert Dempski, Sagar Antala, Elizabeth Bafaro
The Role Of Histidine Residues In The Specificity Of The Human Zinc Transporter Hzip4, Robert Dempski, Sagar Antala, Elizabeth Bafaro
Robert E. Dempski
ZIP transporters, named after the zinc regulated (Zrt) and iron regulated (Irt) transport proteins, are essential for zinc and iron translocation across cellular membranes. These proteins function to increase the cytosolic concentration of transition metals. While both zinc and iron are essential micronutrients which are required for the structure and/or function of hundreds of cellular proteins, the molecular mechanism of ZIP transporters is not well understood. Complicating mechanistic studies is the observation that the concentration of free zinc and iron is nano to picomolar.