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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Characterizing The C-Terminal Region Of Human Adenovirus E1a: An Undiscovered Country, Michael J. Cohen
Characterizing The C-Terminal Region Of Human Adenovirus E1a: An Undiscovered Country, Michael J. Cohen
Electronic Thesis and Dissertation Repository
Human Adenovirus (HAdV) E1A is the first protein expressed during viral infection. The primary function of E1A is to reprogram the cell for viral replication, but it is additionally capable of transforming primary rodent cells in co-operation with other oncogenes such as HAdV E1B. Despite extensive study, little is known about the function and cellular targets of the C-terminal region of E1A. Importantly, this region is required for the transforming ability of E1A with E1B, but can also suppress transformation with Ras. Previous studies showed that interaction with the C-terminal Binding Protein (CtBP) plays a role in both functions described …
Characterizing The Function Of Helix Five Of The Ku70 Von Willebrand A Domain In Non-Homologous End Joining, Sarah M. Hoffer
Characterizing The Function Of Helix Five Of The Ku70 Von Willebrand A Domain In Non-Homologous End Joining, Sarah M. Hoffer
Electronic Thesis and Dissertation Repository
DNA double strand breaks (DSBs) are a toxic and dangerous form of DNA damage repaired primarily by non-homologous end joining (NHEJ) in mammals. The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends. Work in our lab has shown that a D192A/D195R mutation in helix five of the Ku70 von Willebrand A (vWA) domain leads to an NHEJ defect. However, little is known about the function of this region in NHEJ. We hypothesized that helix five of the Ku70 vWA domain mediates a protein-protein interaction that is crucial for …