Biochemistry, Biophysics, and Structural Biology Commons^™

Computational Study Of Ligand-Dependent Oligomerization Of Ribonucleotide Reductase, Bill Pham

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Certain protein oligomerization can be strongly influenced by its ligand-binding status. We constructed a computational method to investigate how ligand-binding and oligomerization can be coupled. We tackle this issue using an approximate approach of studying the properties of individual monomers and how they associate. By connecting the dynamics at monomeric level and the information of oligomer interface, we quantify the synchronization of two types of contact dynamics: (1) between the ligand and its binding pocket, and (2) the contact dynamics at interface. In this work, we applied our methodology on protein ribonucleotide reductase (RNR), which is an essential enzyme for …

Arabidopsis Thaliana Nip2;1 , A Lactic Acid Transporter, Is Essential For Plant Survival During Hypoxic Stress, Samantha Jean Mcintire

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

NIPs are nodulin-intrinsic proteins that are specific to plants and involved in transport of water and other uncharged solutes. Arabidopsis thaliana consists of nine NIPgenes.

In the current study, attention has been focused on NIP2;1(NIPsubgroup I), one of the representative Arabidopsis NIPs, in accordance with its cellular and subcellular localization, tissue, transport activity, and biological roles in Arabidopsis.

It has been shown in experiments that AtNIP2;1is especially responsive to hypoxia-induced stress. Hypoxia is a condition in which most of the oxygen has been removed from an environment. In hypoxic environments, plants construct a …

Constitutive Expression Of Thioglucoside Glucohydrolase 1 (Tgg1) Decreases Intercellular Trafficking In Arabidopsis Thaliana, Alessandro Francesco Sarno

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Plasmodesmata (PD) are pores that traverse plant cell walls, providing a route for intercellular trafficking of essential metabolites, nutrients, and signaling molecules between adjacent plant cells, thereby aiding communication. The increased size exclusion limit 2 (ise2) mutant of Arabidopsis thaliana has an increased abundance of branched PD, as well as a greater flux of intercellular trafficking. A search for proteins that interact with ISE2 identified THIOGLUCOSIDE GLUCOHYDROLASE 2 (a myrosinase). A. thaliana also encodes a second, closely-related myrosinase, TGG1. Myrosinases are enzymes that catalyze the hydrolysis of glucosinolates, a type of secondary metabolite that are amino acid derivatives. The breakdown …