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- Activation energy (1)
- Aminoglycoside Antibiotics (1)
- Aminoglycoside modifying enzymes (1)
- And in vivo (1)
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Articles 1 - 6 of 6
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Environmental Controls On The Diversity And Distribution Of Endosymbionts Associated With Phacoides Pectinatus (Bivalvia: Lucinidae) From Shallow Mangrove And Seagrass Sediments, St. Lucie County, Florida, Thomas Walters Doty
Masters Theses
Lucinid bivalves are capable of colonizing traditionally inhospitable shallow marine sediments due to metabolic functions of bacterial endosymbionts located within their gills. Because lucinids can often be the dominant sediment infauna, defining their roles in sediment and pore fluid geochemical cycling is necessary to address concerns related to changes in coastal biological diversity and to understanding the sensitivity of threatened coastal ecosystems over time. However, there has been limited research done to understand the diversity and distribution of many lucinid chemosymbiotic systems. Therefore, the goals of this thesis were to evaluate the distribution of Phacoides pectinatus and its endosymbiont communities …
Subfunctionalization Of Ethylene Receptors And Homology Modeling Of Cytosolic Domains In Arabidopsis Thaliana, Sai Keerthana Wuppalapati
Subfunctionalization Of Ethylene Receptors And Homology Modeling Of Cytosolic Domains In Arabidopsis Thaliana, Sai Keerthana Wuppalapati
Masters Theses
Ethylene is a gaseous phytohormone that initiates and modulates several mechanisms related to growth and development in plants through a family of five disulphide-linked receptor dimers. Although the ethylene receptors are very similar in their structures, they have diverse functions with both overlapping and non-overlapping roles. Silver ions are able to support ethylene binding to the receptors but it is also interesting to note that ethylene responses are blocked in the presence of silver. A part of the present study identified that ETR1 receiver domain has little or no role in mediating responses to silver ions, supported by data obtained …
The In Vivo Effect Of Osmolytes On Folate Metabolism, Timkhite-Kulu Berhane
The In Vivo Effect Of Osmolytes On Folate Metabolism, Timkhite-Kulu Berhane
Masters Theses
Previous studies have found that addition of osmolytes weakens the binding of dihydrofolate (DHF) to R67 dihydrofolate reductase (DHFR), chromosomal DHFR from E. coli and a pteridine reductase. These results support the preferential interaction of DHF with osmolytes compared to water. Thus, a working model where interaction of DHF with osmolytes shifts the binding away from the protein-DHF complex towards the free species was proposed. As tetrahydrofolate and other folate redox states have similar structures to DHF, we predict osmotic stress will lower the catalytic efficiencies of other folate pathway enzymes. In this thesis, we explore the in vivo effects …
Conformational Dynamics Of Cytochrome P450cam Upon Ligand Binding, Ana Virginia Bernal Gomez
Conformational Dynamics Of Cytochrome P450cam Upon Ligand Binding, Ana Virginia Bernal Gomez
Masters Theses
Cytochrome P450s are a superfamily of metalloenzymes that are responsible for the monooxygenation of their hydrophobic substrates. P450’s retain the same general structural scaffold, however are able to bind promiscuously to substrates with distinct physico-chemical properties. It is believed that they possess considerable flexibility in the substrate binding regions as well as their active site to accomplish the diverse binding and catalytic chemistry with high regio- and stereo-specificity. In order to investigate the conformational dynamics inherent in these enzymes, especially in context of binding different ligands, we carried out amide proton exchange studies via NMR spectroscopy on a model P450 …
Manipulating Adipose Tissue Fatty Acid Oxidation To Reduce Fatness In Broiler Chickens, Tania Emmanuelle Torchon
Manipulating Adipose Tissue Fatty Acid Oxidation To Reduce Fatness In Broiler Chickens, Tania Emmanuelle Torchon
Masters Theses
Compared to rodents, broiler chickens, those reared for meat, are an attractive model for studies of adipose biology, and obesity development in children. The broiler chicken lacks the gene for uncoupling protein 1, the hallmark for brown adipose tissue making them a useful model to study lipid metabolism in white adipocytes. Two studies were performed to investigate if white adipose tissue had the metabolic ability for fatty acid oxidation (FAO), and to investigate the effects of dietary fatty acids on abdominal fat development of young broiler chickens as a model for childhood obesity. In study one, chickens were fasted for …
Comparison Of Activation Enthalpies For Aminoglycoside Modification Reactions, Brittany Sterling Soto
Comparison Of Activation Enthalpies For Aminoglycoside Modification Reactions, Brittany Sterling Soto
Masters Theses
At highly elevated temperatures, many biological reactions can proceed spontaneously from the ground state to the transition state. However, due to the long half-life of these reactions, catalysts are required to catalyze these reactions at modern day temperatures by lowering the activation energy. Wolfenden et al. has previously shown that catalysts enhance the rate of the reaction by reducing the enthalpy of activation. Therefore, the activation energies have been determined for three aminoglycoside modifying enzymes, APH(3’)-IIIa, AAC(3)-IIIb, and AAC(3)-VIa, to determine whether these three enzymes distinguish between the two classes of aminoglycoside antibiotics by reducing the enthalpy of activation during …