Open Access. Powered by Scholars. Published by Universities.®
Biochemistry, Biophysics, and Structural Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Apoptosis (1)
- BMP treatment (1)
- BMPs (1)
- Binding affinity (1)
- Bone mineralization (1)
-
- Bone morphogenetic proteins (1)
- Caspase (1)
- Dissociation constant (1)
- Fluorescence spectroscopy (1)
- Gingivalis (1)
- Glutathione (1)
- Glutathione S-transferase (1)
- Green tea (1)
- Hibernation (1)
- Inflammation (1)
- Isothermal titration calorimetry (1)
- Nonlinear optics (1)
- Nucleoid structuring proteins (1)
- P (1)
- PH dependence (1)
- Partial activation (1)
- Periodontitis (1)
- Periostin (1)
- Periostin gene expression (1)
- Polyphenols (1)
- Shigella (1)
- Supercoiling (1)
- Thermodynamics (1)
- Transcriptional anti-silencing (1)
- Transcriptional silencing (1)
- Publication
- Publication Type
Articles 1 - 7 of 7
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Proliferating Cell Nuclear Antigen Interacts With The Crl4 Ubiquitin Ligase Subunit Cdt2 In Dna Synthesis-Induced Degradation Of Cdt1, Feng Leng, Lovely Saxena, Nam Hoang, Chunxiao Zhang, Logan Lee, Wenjing Li, Xiaoshan Gong, Fei Lu, Hong Sun, Hui Zhang
Proliferating Cell Nuclear Antigen Interacts With The Crl4 Ubiquitin Ligase Subunit Cdt2 In Dna Synthesis-Induced Degradation Of Cdt1, Feng Leng, Lovely Saxena, Nam Hoang, Chunxiao Zhang, Logan Lee, Wenjing Li, Xiaoshan Gong, Fei Lu, Hong Sun, Hui Zhang
Chemistry and Biochemistry Faculty Research
During DNA replication or repair, the DNA polymerase cofactor, proliferating cell nuclear antigen (PCNA), homotrimerizes and encircles the replicating DNA, thereby acting as a DNA clamp that promotes DNA polymerase processivity. The formation of the PCNA trimer is also essential for targeting the replication-licensing protein, chromatin-licensing, and DNA replication factor 1 (CDT1), for ubiquitin-dependent proteolysis to prevent chromosomal DNA re-replication. CDT1 uses its PCNA-interacting peptide box (PIP box) to interact with PCNA, and the CRL4 E3 ubiquitin ligase subunit CDT2 is recruited through the formation of PCNA–CDT1 complexes. However, it remains unclear how CDT1 and many other PIP box–containing proteins …
Ultrafast Laser Filament-Induced Fluorescence Spectroscopy Of Uranyl Fluoride, P. J. Skrodzki, M. Burger, L. A. Finney, F. Poineau, S. M. Balasekaran, J. Nees, K. R. Czerwinski, I. Jovanovic
Ultrafast Laser Filament-Induced Fluorescence Spectroscopy Of Uranyl Fluoride, P. J. Skrodzki, M. Burger, L. A. Finney, F. Poineau, S. M. Balasekaran, J. Nees, K. R. Czerwinski, I. Jovanovic
Chemistry and Biochemistry Faculty Research
Uranyl fluoride (UO2F2) is a compound which forms in the reaction between water and uranium hexafluoride, a uranium containing gas widely used for uranium enrichment. Uranyl fluoride exhibits negligible natural background in atmosphere; as a result, its observation implies the presence and active operation of nearby enrichment facilities and could be used as a tracer for treaty verification technologies. Additionally, detection of UO2F2 has a potential application in guiding remediation efforts around enrichment facilities. Laser-induced fluorescence (LIF) has been proposed in the past as a viable technique for the detection and tracking of UO2F2. We demonstrate that ultrafast laser filamentation …
Dietary Polyphenols And Periodontitis—A Mini-Review Of Literature, Arpita Basu, Emily Masek, Jeffrey L. Ebersole
Dietary Polyphenols And Periodontitis—A Mini-Review Of Literature, Arpita Basu, Emily Masek, Jeffrey L. Ebersole
Kinesiology and Nutrition Sciences Faculty Publications
Periodontitis, which is a chronic infection and disease of the periodontium, is a significant global health burden and is linked to other chronic health conditions such as diabetes and cardiovascular diseases. Dietary polyphenols present in a wide variety of plant-based foods, herbs, and botanicals have been shown to exert antimicrobial, anti-inflammatory, and reduced osteoclast and alveolar bone loss activities in animal models of periodontitis. Polyphenol-containing beverages and foods especially green tea and its active catechin epigallocatechin-3-gallate, cranberries, pomegranates, and fruit and vegetable extracts have reported bacteriostatic/bactericidal activity against microbial species such as P. gingivalis and shown total bacterial burden in …
Transcriptional Silencing And Anti-Silencing Of Virulence Genes In The Bacterial Pathogen Shigella Flexneri: Virb, Dna Supercoiling, And The Histone-Like Nucleoid Structuring Protein, Michael Adam Picker
Transcriptional Silencing And Anti-Silencing Of Virulence Genes In The Bacterial Pathogen Shigella Flexneri: Virb, Dna Supercoiling, And The Histone-Like Nucleoid Structuring Protein, Michael Adam Picker
UNLV Theses, Dissertations, Professional Papers, and Capstones
Transcriptional silencing and anti-silencing affect many aspects of bacterial physiology, including virulence in bacterial pathogens. In Shigella species, a group of gram-negative pathogens that cause bacillary dysentery in humans, the histone-like nucleoid structuring protein (H-NS) transcriptionally silences virulence genes found on the large virulence plasmid while VirB anti-silences these genes. However, the mechanistic details of their interplay are not fully understood. To elucidate their regulatory mechanisms, I use the icsP virulence locus, which shares a long intergenic region with the divergently transcribed ospZ gene (1535 bp from TSS to TSS). Prior to this work, two discrete H-NS binding regions had …
Effect Of Bmp Treatment On Periostin Gene Expression In Pre-Osteoblastic Mc3t3-E1 Mouse Cells, Vincent Lee Khang
Effect Of Bmp Treatment On Periostin Gene Expression In Pre-Osteoblastic Mc3t3-E1 Mouse Cells, Vincent Lee Khang
UNLV Theses, Dissertations, Professional Papers, and Capstones
Periostin is a secreted, extracellular matrix (ECM) protein widely expressed within collagen-rich fibrous connective tissues of the body including the periodontal ligament (PDL), bone, skin, heart, and cornea. Periostin has been shown to serve many important regulatory functions including cell adhesion, cell motility, wound healing and of particular importance to the dental field, differentiation of osteoblasts. The deletion of periostin compromises osteoblast attachment to bone matrix and induces a reduction in mineralization and expression of bone markers, including type I collagen, osteocalcin, osteopontin and alkaline phosphatase. Periostin has also been shown to play a significant role in collagen fibrillogenesis by …
The Caspase Cascade During Hibernation In The Golden-Mantled Ground Squirrel, Spermophilus Lateralis, Michael David Treat
The Caspase Cascade During Hibernation In The Golden-Mantled Ground Squirrel, Spermophilus Lateralis, Michael David Treat
UNLV Theses, Dissertations, Professional Papers, and Capstones
In several human pathologies like heart attack, stroke, neurodegenerative diseases, and autoimmune disorders, widespread cell death, or apoptosis, is a major cause of organ dysfunction and death. Hibernating golden-mantled ground squirrels, Spermophilus lateralis, experience numerous conditions during the winter that are known to be pro-apoptotic in other mammal systems (e.g. extreme hypothermia, ischemia and reperfusion, acidosis, increased reactive oxygen species, bone and muscle disuse). However, studies suggest that hibernators may invoke a protective phenotype to limit widespread cell damage and loss during the hibernation season. Could regulating apoptosis provide protection against the harmful conditions experienced during the hibernation season? Could …
Quantifying The Differences In Binding Affinity Of Reduced Glutathione For Glutathione S-Transferase At Ph 6.5 And 8.5 Using Isothermal Titration Calorimetry, Clay P. Renshaw Jr., Ronald K. Gary
Quantifying The Differences In Binding Affinity Of Reduced Glutathione For Glutathione S-Transferase At Ph 6.5 And 8.5 Using Isothermal Titration Calorimetry, Clay P. Renshaw Jr., Ronald K. Gary
McNair Poster Presentations
The binding affinity between an enzyme and its substrate is often dependent on the pH of the local environment. Knowing the pH at which reduced glutathione (GSH) binds with the highest affinity to the enzyme glutathione S-transferase (GST) is useful for determining the optimal pH for purification of GST-fusion proteins during GST-affinity chromatography. In this study, GST of the species Schistosoma japonicum was purified, quantified, and utilized to study its binding interaction with GSH at pH 6.5 and 8.5 via isothermal titration calorimetry (ITC). After protein expression, extraction, and purification, the GST concentration was quantified using QubitTM fluorometry. …