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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Thermogenic Characteristics Of Adipocytes Are Dependent On The Regulation Of Iron Homeostasis, Jin-Seon Yook, Mikyoung You, Yongeun Kim, Mi Zhou, Zhenhua Liu, Young-Cheul Kim, Jaekwon Lee, Soonkyu Chung
The Thermogenic Characteristics Of Adipocytes Are Dependent On The Regulation Of Iron Homeostasis, Jin-Seon Yook, Mikyoung You, Yongeun Kim, Mi Zhou, Zhenhua Liu, Young-Cheul Kim, Jaekwon Lee, Soonkyu Chung
Nutrition Department Faculty Publication Series
The development of thermogenic adipocytes concurs with mitochondrial biogenesis, an iron-dependent pathway. Iron regulatory proteins (IRP) 1 and 2 are RNA-binding proteins that regulate intracellular iron homeostasis. IRPs bind to the iron-response element (IRE) of their target mRNAs, balancing iron uptake and deposition at the posttranscriptional levels. However, IRP/IRE-dependent iron regulation in adipocytes is largely unknown. We hypothesized that iron demands are higher in brown/beige adipocytes than white adipocytes to maintain the thermogenic mitochondrial capacity. To test this hypothesis, we investigated the IRP/IRE regulatory system in different depots of adipose tissue. Our results revealed that 1) IRP/IRE interaction was increased …
Modulating Glutathione Thiol Status Alters Pancreatic Β-Cell Morphogenesis In The Developing Zebrafish (Danio Rerio) Embryo, Archit Rastogi, Emily G. Severance, Haydee M. Jacobs, Sarah M. Conlin, Sadia T. Islam, Alicia R. Timme-Laragy
Modulating Glutathione Thiol Status Alters Pancreatic Β-Cell Morphogenesis In The Developing Zebrafish (Danio Rerio) Embryo, Archit Rastogi, Emily G. Severance, Haydee M. Jacobs, Sarah M. Conlin, Sadia T. Islam, Alicia R. Timme-Laragy
Environmental Health Sciences Faculty Publication Series
Emerging evidence suggests that redox-active chemicals perturb pancreatic islet development. To better understand potential mechanisms for this, we used zebrafish (Danio rerio) embryos to investigate roles of glutathione (GSH; predominant cellular redox buffer) and the transcription factor Nrf2a (Nfe2l2a; zebrafish Nrf2 coortholog) in islet morphogenesis. We delineated critical windows of susceptibility to redox disruption of beta-cell morphogenesis, interrogating embryos at 24, 48 and 72 h post fertilization (hpf) and visualized Nrf2a expression in the pancreas using whole-mount immunohistochemistry at 96 hpf. Chemical GSH modulation at 48 hpf induced significant islet morphology changes at 96 hpf. Pro-oxidant exposures to tert-butylhydroperoxide (77.6 …