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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Distribution Of Limonin During The Growth And Development Of Leaves And Branches Of Citrus Paradisi, Cecilia Mcintosh, Richard L. Mansell Feb 1983

Distribution Of Limonin During The Growth And Development Of Leaves And Branches Of Citrus Paradisi, Cecilia Mcintosh, Richard L. Mansell

Cecilia A. McIntosh

This report analyzes the distribution of limonin within a leaf, within a branch, and between branches of an individual Citrus paradisi tree.  Limonin was evenly distributed within single leaves and the concentration in the leaves on a given branch was constant, regardless of branch age.  The total amount of limonin per leaf varied with leaf size.  Not all branches within the tree exhibited the same potential for limonin metabolism as reflected in their limonin and limonoate A-ring monolactone levels.  The limonin content showed increasing levels as the leaves bud and grow and then decreasing levels as they mature and turn …


Monoclonal Antibodies Reveal The Structural Basis Of Antibody Diversity, Jean-Luc Teillaud, Catherine Desaymard, Angela Giusti, Barbara Haseltine, Roberta Pollock, Dale Yelton, Donald Zach, Matthew Scharff Dec 1982

Monoclonal Antibodies Reveal The Structural Basis Of Antibody Diversity, Jean-Luc Teillaud, Catherine Desaymard, Angela Giusti, Barbara Haseltine, Roberta Pollock, Dale Yelton, Donald Zach, Matthew Scharff

Roberta Pollock

Hybridoma technology has made it possible to introduce into continuous culture normal antibody-forming cells and to obtain large amounts of the immunoglobulin produced by each of these cells. Examination of the structure of a number of monoclonal antibodies that react with a single antigen has provided new information on the structural basis of the specificity and affinity of antibodies. Comparisons of families of monoclonal antibodies derived from a single germ line gene revealed the importance of somatic mutation in generating antibody diversity. Monoclonal antibodies that react with variable regions of other monoclonals allow the further dissection and modulation of the …


An Analysis Of The Limonin And Naringin Content Of Grapefruit Juice Samples Collected From Florida State Test Houses, Richard L. Mansell, Cecilia Mcintosh, Susan E. Vest Dec 1982

An Analysis Of The Limonin And Naringin Content Of Grapefruit Juice Samples Collected From Florida State Test Houses, Richard L. Mansell, Cecilia Mcintosh, Susan E. Vest

Cecilia A. McIntosh

An analysis of the limonin and naringin content of 6685 grapefruit juice samples (representing approximately 6% of all grapefruit harvested) collected from three Florida State Test Houses showed that there were statistically significant differences between the Test Houses.  There also was a statistically significant difference in the concentration of these compounds in the juice from the different cultivars of fruit sampled.  Average limonin and naringin concentrations in the juice remained fairly constant through the beginning of the season until a freeze occurred in early 1981.  Juice obtained from fruit harvested after this freeze contained decreasing amounts of limonin and increasing …