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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Structural Adaptations Of Proteins To Different Biological Membranes, Prof. Stephanie Tristram-Nagle, Irina Pogozheva, Harold Mosberg, Andrei Lomize
Structural Adaptations Of Proteins To Different Biological Membranes, Prof. Stephanie Tristram-Nagle, Irina Pogozheva, Harold Mosberg, Andrei Lomize
Prof. Stephanie Tristram-Nagle Ph.D.
To gain insight into adaptations of proteins to their membranes, intrinsic hydrophobic thicknesses, distributions of different chemical groups and profiles of hydrogen-bonding capacities (α and β) and the dipolarity/ polarizability parameter (π*) were calculated for lipid-facing surfaces of 460 integral α-helical, β-barrel and peripheral proteins from eight types of biomembranes. For comparison, polarity profiles were also calculated for ten artificial lipid bilayers that have been previously studied by neutron and X-ray scattering. Estimated hydrophobic thicknesses are 30–31 Å for proteins from endoplasmic reticulum, thylakoid, and various bacterial plasma membranes, but differ for proteins from outer bacterial, inner mitochondrial and eukaryotic …
Membrane Structure Correlates To Function Of Llp2 On The Cytoplasmic Tail Of Hiv-1 Gp41 Protein, Alexander Boscia, Zachary Benamram, Jonathan Michel, Michael Jablin, Jonathan D. Steckbeck, Ronald C. Montelaro, John F. Nagle, Prof. Stephanie Tristram-Nagle Ph.D.
Membrane Structure Correlates To Function Of Llp2 On The Cytoplasmic Tail Of Hiv-1 Gp41 Protein, Alexander Boscia, Zachary Benamram, Jonathan Michel, Michael Jablin, Jonathan D. Steckbeck, Ronald C. Montelaro, John F. Nagle, Prof. Stephanie Tristram-Nagle Ph.D.
Prof. Stephanie Tristram-Nagle Ph.D.
Mutation studies previously showed that the lentivirus lytic peptide (LLP2) sequence of the cytoplasmic C-terminal tail of the HIV-1 gp41 envelope protein inhibited viral-initiated T-cell death and T-cell syncytium formation, at which time in the HIV life cycle the gp41 protein is embedded in the T-cell membrane. In striking contrast, the mutants did not affect virion infectivity, during which time the gp41 protein is embedded in the HIV envelope membrane. To examine the role of LLP2/membrane interactions, we applied synchrotron X-radiation to determine structure of hydrated membranes. We focused on WT LLP2 peptide (þlus three charge) and MX2 mutant (negative …