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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
In Vivo Tissue-Specific Chromatin Profiling In Drosophila Melanogaster Using Gfp-Tagged Nuclei, Juan Jauregui-Lozano, Kimaya Bakhle, Vikki M. Weake
In Vivo Tissue-Specific Chromatin Profiling In Drosophila Melanogaster Using Gfp-Tagged Nuclei, Juan Jauregui-Lozano, Kimaya Bakhle, Vikki M. Weake
Department of Biochemistry Faculty Publications
The chromatin landscape defines cellular identity in multicellular organisms with unique patterns of DNA accessibility and histone marks decorating the genome of each cell type. Thus, profiling the chromatin state of different cell types in an intact organism under disease or physiological conditions can provide insight into how chromatin regulates cell homeostasis in vivo. To overcome the many challenges associated with characterizing chromatin state in specific cell types, we developed an improved approach to isolate Drosophila melanogaster nuclei tagged with a GFPKASH protein. The perinuclear space-localized KASH domain anchors GFP to the outer nuclear membrane, and expression of UAS-GFPKASH can …
Quantitative Proteomic And Metabolomic Profiling Reveals Altered Mitochondrial Metabolism And Folate Biosynthesis Pathways In The Aging Drosophila Eye, Hana Hall, Bruce R. Cooper, Guihong Qi, Aruna B. Wijeratne, Amber L. Mosley, Vikki M. Weake
Quantitative Proteomic And Metabolomic Profiling Reveals Altered Mitochondrial Metabolism And Folate Biosynthesis Pathways In The Aging Drosophila Eye, Hana Hall, Bruce R. Cooper, Guihong Qi, Aruna B. Wijeratne, Amber L. Mosley, Vikki M. Weake
Department of Biochemistry Faculty Publications
Aging is associated with increased risk of ocular disease, suggesting that age-associated molecular changes in the eye increase its vulnerability to damage. Although there are common pathways involved in aging at an organismal level, different tissues and cell types exhibit specific changes in gene expression with advanced age. Drosophila melanogaster is an established model system for studying aging and neurodegenerative disease that also provides a valuable model for studying age-associated ocular disease. Flies, like humans, exhibit decreased visual function and increased risk of retinal degeneration with age. Here, we profiled the aging proteome and metabolome of the Drosophila eye and …