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Georgia Southern University

Osmotic sensitive

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Characterization And Genetic Analysis Of A Pigmentation Mutant Of Neurospora Crassa, Craig West Banks Jan 1994

Characterization And Genetic Analysis Of A Pigmentation Mutant Of Neurospora Crassa, Craig West Banks

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The mutant overaccumulator of carotenoids, ovc. was obtained from the Fungal Genetics Stock Center in Kansas City, Kansas. This mutant had been isolated following UV irradiation of the colonial mutant col-4 and mapped to Linkage Group IV (R. Harding, 1984 Neurospora Newsletter 34: 23-25). Examination of ovc in our laboratory revealed that in addition to having increased pigmentation, the mutant was osmotic sensitive, failing to grow on 6% NaCl. Morphology of ovc closely resembles that of cut, another osmotic sensitive mutant in LG IV. A cross of ovc to the osmotic sensitive mutant cut failed to produce …

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Isolation And Characterization Of Vinclozolin-Resistant Mutants Of Neurospora Crassa, Kimberly Booth Thurmon Jan 1987

Isolation And Characterization Of Vinclozolin-Resistant Mutants Of Neurospora Crassa, Kimberly Booth Thurmon

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Six vinclozolin-resistant mutants of N. crassa obtained following ultraviolet irradiation and selection on vinclozolin media, showed 1:1 segregation of the vinclozolin-resistant trait. In addition to being vinclozolin resistant, the six mutants had altered, non-wild-type morphology and were osmotic sensitive, with the different mutants exhibiting varying degrees of sensitivity. Genetic analysis indicated linkage of the three traits. Reappearance of osmotic sensitivity/altered morphology in crosses of phenotypically recombinant F3 progeny supported the presence of modifier(s). Rates of growth on vinclozolin media placed the six mutants into three groups. In most cases the rates were comparable to rates of growth on W-M …

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