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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Alteration In The Expression Of Compartmentation: In Vitro Studies / D. D. Clarke And S. Berl, Donald Dudley Clarke Phd, Soll Berl
Alteration In The Expression Of Compartmentation: In Vitro Studies / D. D. Clarke And S. Berl, Donald Dudley Clarke Phd, Soll Berl
Chemistry Faculty Publications
Glutamic acid and glutamine are present in unusually large concentrations in brain. The only known enzymatic mechanism which has been reported for the synthesis of glutamine is that catalysed by glutamine synthetase (E.C.6.3.1.2). These amino acids provide a very useful probe for studying precursor-product relationships in brain tissue in vivo. It is evident that the non-steady state situation presented by injection of a single dose of inhibitor followed by a tracer dose of a labelled precursor at some later time-a practice which has been used extensively in previous attempts to clarify the effects of metabolic inhibitors-makes it rather difficult to …
Effects Of Li+ On The Metabolism In Brain Of Glutamate, Glutamine, Aspartate And Gaba From [I-14]Acetate In Vitro / S. Berl And D. D. Clarke Department Of Neurology, College Of Physicians And Surgeons, Columbia University, New York, N.Y. 10032 And Chemistry Department, Fordham University, Bronx, N.Y. 10458 (U.S.A.), Soll Berl, Donald Dudley Clarke Phd
Effects Of Li+ On The Metabolism In Brain Of Glutamate, Glutamine, Aspartate And Gaba From [I-14]Acetate In Vitro / S. Berl And D. D. Clarke Department Of Neurology, College Of Physicians And Surgeons, Columbia University, New York, N.Y. 10032 And Chemistry Department, Fordham University, Bronx, N.Y. 10458 (U.S.A.), Soll Berl, Donald Dudley Clarke Phd
Chemistry Faculty Publications
Guinea pig brain slices were incubated in media in which Li+ replaced part of the Na+. Leakage of glutamic acid, glutamine, aspartic acid and GABA into the media were increased markedly; glutamic acid was most affected. The uptake of [1-14C]acetate was decreased in the presence of Li+. Of the amino acids related to the citric acid cycle the labeling of glutamine was most affected. The presence of 100% O2 counteracts in part the Li+ effects. The results were interpretable on the basis of the compartmentation of citric acid cycle metabolism in brain. It is suggested that the pool of 'transmitter' …