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Articles 1 - 7 of 7
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Cooperativity And Competition In The Binding Of Intercalators And Groove Binders To Dna, Nidia Rodriguez
Cooperativity And Competition In The Binding Of Intercalators And Groove Binders To Dna, Nidia Rodriguez
Undergraduate Research at FIU (URFIU) Conference
Both DNA intercalating ligands such as psoralens and DNA groove binding ligands such as heterocyclic diamidines have been shown to alter the activity of restriction endonucleases if bound at or near the restriction enzyme reaction site. When two agents have binding sites near a particular restriction enzyme site, the possibility arises that each may affect the binding and binding consequences of the other. We have examined this by examining the effects of heterocyclic diamidine mixtures or of diamidine/psoralen mixtures or of heterocyclic diamidine/Co+2 mixtures upon the reactivity of several restriction enzymes on phix174RF DNA. For example, with the restriction enzyme …
Exploring The Conformational Space Of Growth Hormone - Releasing Hormone Analogs Using Dopant Assisted Trapped Ion Mobility Spectrometry - Mass Spectrometry, Javier Moreno
Undergraduate Research at FIU (URFIU) Conference
Recently, we proposed a high-throughput screening workflow for the elucidation of agonistic or antagonistic growth hormone – releasing hormone (GHRH) potencies based on structural motif descriptors as a function of the starting solution. In the present work, we revisited the influence of solution and gas-phase GHRH molecular micro-environment using trapped ion mobility – mass spectrometry (TIMS-MS). The effect of the starting solvent composition (10 mM ammonium acetate (NH4Ac), 50% methanol (MeOH), 50% acetonitrile (MeCN) and 50% acetone (Ac)) and gas-phase modifiers (N2 , N2 +MeOH, N2 +MeCN and N2 +Ac) on the conformational states of three GHRH analogs, GHRH (1-29), …
Mechanistic Studies Of Mycobacterium Tuberculosis Topoisomerase 1 Inhibition By Endogenous Toxin Rv1495, Rosemarie Martinez Borrero
Mechanistic Studies Of Mycobacterium Tuberculosis Topoisomerase 1 Inhibition By Endogenous Toxin Rv1495, Rosemarie Martinez Borrero
Undergraduate Research at FIU (URFIU) Conference
Antimicrobial resistance is a worldwide public health concern as existing antibiotics are becoming ineffective. Tuberculosis is the leading infectious cause of death worldwide. According to the World Health Organization, in 2017, there were 558,000 cases of drug resistant tuberculosis (TB), so there is an urgent need of new TB treatment against a novel target. Toxin-antitoxin (TA) systems participate as gene regulators within bacteria. Researchers believe that TA systems contribute to the long-term dormancy of Mycobacterium tuberculosis (Mtb) within the host-cell environment. Previous research showed that an Mtb toxin Rv1495, a homolog of MazF that is part of the MazEF TA …
Interactions Of Neuronal Calcium Sensor Dream With Zinc, Maria J. Santiago
Interactions Of Neuronal Calcium Sensor Dream With Zinc, Maria J. Santiago
Undergraduate Research at FIU (URFIU) Conference
Downstream regulatory element antagonist modulator (DREAM) is an EF-hand protein that is highly expressed in the central nervous system in areas such as the hippocampus and the neural cortex and regulates kinetics of potassium channels, gene expression, calcium homeostasis and enzymatic activity of presenilin. Characterization of the metal-binding properties of DREAM and how it affects DEAM structure and DREAM interactions with effector proteins are crucial to understand its biological function under physiological and pathological conditions. Essential metals like Mg2+, Zn2+ and Ca2+ play extremely important roles in biological processes through direct interactions with proteins. DREAM carry …
Analysis Of Hydroxylated Polychlorinated Biphenyls Using Nesi-Tims-Ms, Natalie Smith
Analysis Of Hydroxylated Polychlorinated Biphenyls Using Nesi-Tims-Ms, Natalie Smith
Undergraduate Research at FIU (URFIU) Conference
Polychlorinated biphenyls (PCBs) are industrial pollutants classified as endocrine disruptors and can cause antagonistic effects in the reproductive, neurological, and immunity systems in humans and animals. The biological toxicity of PCBs depends on the position and number of chlorine atoms within the molecule, and they work to mimic or block essential hormones, causing significant health damages. In the present work, nine different congeners of hydroxylated PCBs (OH-PCBs), with varying number of chlorine atoms, were studied using trapped ion mobility coupled to mass spectrometry (TIMS-MS). In particular, isomers of OH-hepta-chlorinated biphenyls (e.g., 4-OH-PCB 187, 4-OH-PCB 172 and 3-OH-PCB 180) were analyzed …
A Rapid And Sensitive High-Throughput Screening Method To Identify Compounds Targeting Protein–Nucleic Acids Interactions, Nicole Alonso, Roboan Guillen
A Rapid And Sensitive High-Throughput Screening Method To Identify Compounds Targeting Protein–Nucleic Acids Interactions, Nicole Alonso, Roboan Guillen
Undergraduate Research at FIU (URFIU) Conference
DNA-binding and RNA-binding proteins are usually considered ‘undruggable’ partly due to the lack of an efficient method to identify inhibitors from existing small molecule repositories. Here we report a rapid and sensitive high-throughput screening approach to identify compounds targeting protein–nucleic acids interactions based on protein–DNA or protein–RNA interaction enzyme-linked immunosorbent assays (PDI-ELISA or PRI-ELISA). We validated the PDI-ELISA method using the mammalian highmobility- group protein AT-hook 2 (HMGA2) as the protein of interest and netropsin as the inhibitor of HMGA2–DNA interactions. With this method we successfully identified several inhibitors and an activator for HMGA2–DNA interactions from a collection of 29 …
Conformational Kinetics Study Of Mp-11 Using Tims-Ms And Molecular Dynamics, Christopher Harrilal, Jaroslava Miksovska, Emily R. Schenk
Conformational Kinetics Study Of Mp-11 Using Tims-Ms And Molecular Dynamics, Christopher Harrilal, Jaroslava Miksovska, Emily R. Schenk
Undergraduate Research at FIU (URFIU) Conference
In the present work, we studied the conformational kinetics of microperoxidase 11 (MP-11), a digest fragment of cytochrome C that contains 11 amino acids with a covalently attached heme group. In particular, a novel technique recently developed at FIU in collaboration with Bruker Daltonics Inc. combined with theoretical calculation was used for the characterization of MP- 11 conformational space [1-3]. Accurate ion-neutral collision cross sections were measured for all MP-11 generated charge states. Since MP-11 (like cytochrome C) undergoes conformational changes as a function of the solvent state, MP-11 ions were produced by electrospray ionization (ESI) in order to preserve …