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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
All Theses
A method for the isolation of intact physiologically active plastids from rapidly developing soybean (Glycine max L.) somatic embryos has been developed for the in vitro study of lipid metabolism. Using de novo fatty acid biosynthesis from 14C-acetate as a marker for physiological functionality, the greatest rates of fatty acid biosynthesis were recovered in 3000 x g fractions that were isolated in the presence of 0.5 M sorbitol, with essentially no activity occurring in the 3000 x g supernatant. Plastids purified on 10% Percoll were approximately 70 and 97 % free from mitochondrial and ER contamination, respectively, as judged …
Changes In Expression Of Akt Pathway Proteins Following Treatment With Rg3 In Vitro, Kathryn Schalkoff
Changes In Expression Of Akt Pathway Proteins Following Treatment With Rg3 In Vitro, Kathryn Schalkoff
All Theses
To assess changes in AKT pathway signaling, a recombinant protein of the G3 domain of rat laminin-5 (rG3) that specifically binds the alpha subunit of integrins α6β1 and α6β4 expressed on cancer cells (e.g., MDA-MB-231) was produced. This recombinant protein is believed to interrupt the intracellular signaling events of the AKT pathway, causing a decrease in proliferation and survival of cells after treatment. Viability assays confirmed an apoptotic effect of rG3 on cells in a dose-dependent manner. However, data from gene expression studies of Caspase-9, GRB10, and CDKNIB proved non-conclusive that rG3 is acting upon gene expression, leading to the …
Biomass V2.0: A New Tool For Bioprocess Simulation, Y Phan-Thien
Biomass V2.0: A New Tool For Bioprocess Simulation, Y Phan-Thien
All Theses
A simulation software (BioMASS - Biological Modeling and Simulation Software) was upgraded from the previous version and provided with additional enhancements. Several new bioprocess configurations and their subroutines have been added. The additional processes included continuous stirred tank reactor (CSTR) with biomass recycle, and CSTR with additional stream in the second stage. The primary goal in the development of BioMASS v2.0 was to provide users with a ready-to-use, expressive visual modeling tools. In this new version, output from simulation can be visualized in graphics. Printing, exporting, and saving file options also are available. In summary, BioMASS v2.0 offers an effective …