Biochemistry, Biophysics, and Structural Biology Commons^™

Structural Characterization Of The Redox-Dependent Differences In The Cytochrome P450cam-Putidaredoxin Complex Using Solution Nmr Spectroscopy, Nicholas John Lopes

Masters Theses

Complexation between proteins as part of biological electron transfer reactions is driven by precise interactions that are often characterized by short lifetimes, weak affinities and high turnover rates. These complex interactions are difficult to study structurally in physiologically relevant oxidation states due to their transient nature and/or large molecular sizes. One such protein complex in the cytochrome P450 family of enzymes that is of great interest to researchers due to its prototypical nature is the Putidaredoxin (Pdx)- cytochrome P450cam (CYP101) electron transfer complex that is involved in hydroxylation of D-camphor in the bacterium Pseudomonas putida. While the individual protein structures …

Nuclear Magnetic Resonance Spectroscopic And Computational Investigations Of Chloroperoxidase Catalyzed Regio- And Enantio-Selective Transformations, Rui Zhang

FIU Electronic Theses and Dissertations

Chloroperoxidase (CPO) is the most versatile heme-containing enzyme that catalyzes a broad spectrum of reactions. The remarkable feature of this enzyme is the high regio- and enantio-selectivity exhibited in CPO-catalyzed oxidation reactions. The aim of this dissertation is to elucidate the structural basis for regio- and enantio-selective transformations and investigate the application of CPO in biodegradation of synthetic dyes.

To unravel the mechanism of CPO-catalyzed regioselective oxidation of indole, the dissertation explored the structure of CPO-indole complex using paramagnetic relaxation and molecular modeling. The distances between the protons of indole and the heme iron revealed that the pyrrole ring of …

Utilizing Nmr Spectroscopy And Molecular Docking As Tools For The Structural Determination And Functional Annotation Of Proteins, Jaime Stark

Department of Chemistry: Dissertations, Theses, and Student Research

With the completion of the Human Genome Project in 2001 and the subsequent explosion of organisms with sequenced genomes, we are now aware of nearly 28 million proteins. Determining the role of each of these proteins is essential to our understanding of biology and the development of medical advances. Unfortunately, the experimental approaches to determine protein function are too slow to investigate every protein. Bioinformatics approaches, such as sequence and structure homology, have helped to annotate the functions of many similar proteins. However, despite these computational approaches, approximately 40% of proteins still have no known function. Alleviating this deficit will …