Biochemistry, Biophysics, and Structural Biology Commons^™

Drought Responsive Gene Expression Regulatory Divergence Between Upland And Lowland Ecotypes Of A Perennial C4 Grass, John T. Lovell, Scott Schwartz, David B. Lowry, Eugene V. Shakirov, Jason E. Bonnette, Xiaoyu Weng, Mei Wang, Jenifer Johnson, Avinash Sreedasyam, Christopher Plott, Jerry Jenkins, Jeremy Schmutz, Thomas E. Juenger

Yevgeniy (Eugene) Shakirov

Climatic adaptation is an example of a genotype-by-environment interaction (G×E) of fitness. Selection upon gene expression regulatory variation can contribute to adaptive phenotypic diversity; however, surprisingly few studies have examined how genome-wide patterns of gene expression G×E are manifested in response to environmental stress and other selective agents that cause climatic adaptation. Here, we characterize drought-responsive expression divergence between upland (drought-adapted) and lowland (mesic) ecotypes of the perennial C₄ grass, Panicum hallii, in natural field conditions. Overall, we find that cis-regulatory elements contributed to gene expression divergence across 47% of genes, 7.2% of which exhibit drought-responsive G×E. …

Phospholipase Dζ Enhances Diacylglycerol Flux Into Triacylglycerol, Wenyu Yang, Geliang Wang, Jia Li, Philip David Bates, Xuemin Wang, Douglas K. Allen

Xuemin (Sam) Wang

Identification And Metabolite Profiling Of Chemical Activators Of Lipid Accumulation In Green Algae, Nishikant Wase, Boqiang Tu, Paul N. Black, Concetta C. Dirusso

Nishikant Wase

Non-Specific Phospholipase C1 Affects Silicon Distribution And Mechanical Strength In Stem Nodes Of Rice, Huasheng Cao, Lin Zhuo, Yuan Su, Linxiao Sun, Xuemin Wang

Xuemin (Sam) Wang

Expression Of Acyl Carrier Proteins In Spinach, Katherine Schmid, J. Ohlrogge

Katherine Schmid

Dr. Schmid and Dr. Ohlrogge's contribution to the Proceedings of the 9th International Symposium on Plant Lipids; Wye, England; July 8-13, 1990.

Dihydrosterculate In Tobacco Transformed With Bacterial Cyclopropane Fatty Acid Synthase, Katherine Schmid

Katherine Schmid

Many gram negative bacteria accumulate cyclopropane fatty acids (CPFAs) in their membranes during stationary phase. In plants, on the other hand, CPFAs are best known as constituents of certain seed oils. CPFAs make up around 40% of Litchi chinensis seed oil [1] and a significant proportion of oils from other Sapindales. In order Malvales, small amounts of CPFA typically accompany cyclopropene fatty acids in seed oils, although up to 5% CPFA has been observed in the polar lipid fraction from Malvaceous roots [2]. Genetic engineering of oilseeds for unusual fatty acid production requires that the introduced fatty acids accumulate in …

Cyclopropane Fatty Acid Expression In Plants, Katherine Schmid

Katherine Schmid

Pants [sic] are transformed with a bacterial cyclopropane fatty acid synthase gene to produce lipids containing cyclopropane fatty acids. Using this technology dihydrosterculate is produced in oilseed crops such as rape.

Membrane Glycerolipidome Of Soybean Root Hairs And Its Response To Nitrogen And Phosphate Availability, Fang Wei, Brian Fanella, Liang Guo, Xuemin Wang

Xuemin (Sam) Wang

The Reversible Splitting Of Organomercuric Cyanides With Hydrogen Chloride , Edward Carr

Edward R. Carr

No abstract provided.

The Role Of Protein Kinases Rog1 And Srf6 In The Wak Stress Response Pathway, Jaepil Eric Yoon

Jaepil Eric Yoon

The wall-associated kinases (WAKs) are receptor kinases that bind cell wall pectin to activate expansion and stress response pathways in Arabidopsis thaliana. When bound to pectin polymers, WAKs initiate expansion by inducing the expression of vacuolar invertases that increase cellular turgor pressure, which pushes out against the cell wall. WAKs also bind pathogen or wounding induced pectin fragments called oligogalacturonic acids (OGs). When bound to OGs, WAKs induce a stress response, which leads to the activation of MAP kinases, MPK3 and MPK6. Phosphoproteomics and mass spectrometry identified receptor kinase SRF6, cytoplasmic kinase ROG1, and nuclear DNA binding protein REM2 to …

Phytoconstituents Analysis, Antioxidant Capacity And Antimicrobial Properties Of Extracts From Laggera Aurita L. (Asteraceae), Crépin I Dibala, Kiessoun Konaté, Mamounata Diao, Maurice Ouédraogo, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Objective: This research was designed to evaluate the polyphenolic content, antibacterial potency and antioxidant activity of extract and fractions from Laggera aurita L. (Asteraceae), a medicinal herbaceous from Burkina Faso. Methods: Folin ciocalteu and AlCl3 methods respectively were used for polyphenol contents. The antioxidant activities of the samples were evaluated by various in vitro assays like ferrous reducing antioxidant assay (FRAP), Total reducing power, 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ABTS radical cation decolorization assays. In vitro antibacterial capacity of bioactive fractions were investigated by agar disc diffusion, micro-well dilution (MIC), Minimum Bactericidal Concentration (MBC) assay and at least the effect …

Purification And Characterization Of Sweet Potato (Ipomoea Batatas) Peroxidase, Mamouna Diao, Brice N’Cho Ayékoué, Crépin I. Dibala, Soumaïla Dabonné, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Peroxidase (POX), a biotechnologically important enzyme was purified from bulb of Ipomoea batatas and characterized for use in the bioconversion of phenolic compounds from industrial wastewater. Purified peroxidase (POXp) was obtained using the gel-filtration chromatography and anion exchange chromatography. A final yield of 18 % was obtained with 20.5 as purification fold. Electrophoresis on SDS-PAGE and native-PAGE showed that POXp is monomeric with a molecular weight of 44 kDa. The optimum pH and optimum temperature were respectively 6.0 and 45 ° C. All enzyme activity was retained for 2 hours at pH values between 4.0 to 9.0. While the enzyme …

Current State Of Food Security: Research, Education And Outreach In Burkina Universities, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Burkina Faso is a Sahelian West African country. Current population is about 16 millions and 43.9 % live below the poverty line. Burkina Faso is classified as both a least developed country and a low-income, food-deficit country. The nutritional quality is poor (1830 against 2000 Kcal/person/day). Food insecurity affects 50 % of households, and is increasing in the urban areas. However, Burkina Faso is one of the country that respect the 2003 Maputo’s declaration’s call to invest at least 10 percent of the budget in agriculture, in order to increase agricultural production and attain food self-sufficiency. Several training programs dealing …

Synthesis, Linda H. Pardo, Linda H. Geiser, Mark E. Fenn, Charles T. Driscoll, Christine L. Goodale, Edith B. Allen, Jill S. Baron, Roland Bobbink, William D. Bowman, Christopher M. Clark, Bridget Emmett, Frank S. Gilliam, Tara Greaver, Sharon J. Hall, Erik A. Lilleskov, Lingli Liu, Jason A. Lynch, Knute Nadelhoffer, Steven S. Perakis, Molly J. Robin-Abbott, John L. Stoddard, Kathleen C. Weathers

Frank S. Gilliam

Human activity in the last century has led to a substantial increase in nitrogen (N) emissions and deposition. This N deposition has reached a level that has caused or is likely to cause alterations to the structure and function of many ecosystems across the United States. One approach for quantifying the level of pollution that would be harmful to ecosystems is the critical loads approach. The critical load is dei ned as the level of a pollutant below which no detrimental ecological effect occurs over the long term according to present knowledge. The objective of this project was to synthesize …

Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle Landry

Kyle S Landry

A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a genomic database and was referred to as TM-417. TM-417 was found to have an optimal growth temperature of 45 ^oC and an optimal pH of 7.0. An extracellular DNase and RNase was found to be produced by the isolate and were purified 145.58-fold and 127.6-fold respectively using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of purification was determined …

List Of Thylakoid Zinc-Finger Proteins In Arabidopsis, Yan Lu

Yan Lu

No abstract provided.

The Occurrence Of A Thylakoid-Localized Small Zinc Finger Protein In Land Plants, Yan Lu

Yan Lu

No abstract provided.

A Small Zinc Finger Thylakoid Protein Plays A Role In Maintenance Of Photosystem Ii In Arabidopsis Thaliana, Yan Lu, David Hall, Robert Last

Yan Lu

No abstract provided.

Chloroplast 2010: A Database For Large-Scale Phenotypic Screening Of Arabidopsis Mutants, Yan Lu, Linda Savage, Matthew Larson, Curtis Wilkerson, Robert Last

Yan Lu

No abstract provided.

Chloroplast Phenomics: Systematic Phenotypic Screening Of Chloroplast Protein Mutants In Arabidopsis, Yan Lu, Linda Savage, Robert Last

Yan Lu

No abstract provided.

Small Rnas Prevent Transcription-Coupled Erosion Of Histone H3 Lysine 9 Methylation In Arabidopsis Thaliana, Raymond Enke, Z. Dong, J. Bender

Ray Enke Ph.D.

No abstract provided.

Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last

Yan Lu

No abstract provided.

Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last

Yan Lu

No abstract provided.

New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last

Yan Lu

No abstract provided.

The Importance Of Maltose In Transitory Starch Breakdown, Yan Lu, Thomas Sharkey

Yan Lu

No abstract provided.

Cellular And Organ Level Localization Of Maltose In Maltose-Excess Arabidopsis Mutants, Yan Lu, Jon Steichen, Sean Weise, Thomas Sharkey

Yan Lu

No abstract provided.

The Role Of Cytosolic A-Glucan Phosphorylase In Maltose Metabolism And The Comparison Of Amylomaltose In Arabidopsis And E. Coli, Yan Lu, Jon Steichen, Jian Yao, Thomas Sharkey

Yan Lu

No abstract provided.

Food Based Approaches For A Healthy Nutrition In Africa, Mamoudou Hama Dicko

Pr. Mamoudou H. DICKO, PhD

The latest estimates of the FAO demonstrate the problems of the fight against hunger. These problems are manifested by the ever-increasing number of chronically undernourished people worldwide. Their numbers during the 1999-2001 period were estimated at about 840 million of which 798 million live in developing countries. Sub-Saharan Africa alone represented 198 million of those. In this part of Africa the prevalence of undernourishment ranges from 5-34%, causing growth retardation and insufficient weight gain among one third of the children under five years of age and resulting in a mortality of 5-15% among these children. Malnutrition resulting from undernourishment is …

The Role Of Amylomaltase In Maltose Metabolism In The Cytosol Of Photosynthetic Cells, Yan Lu, Thomas Sharkey

Yan Lu

No abstract provided.

Action Of Heptaminol Hydrochloride On Contractile Properties In Frog Isolated Twitch Muscle Fibre, Bruno Allard, Vincent Jacquemont, Fouad Lemtiri-Chlieh, Bernard Pourrias, Oger Rougier

fouad Lemtiri-Chlieh

1 Heptaminol stopped or delayed the progressive decline in tension which characterizes the phenomenon of fatigue in frog isolated twitch muscle fibre. 2 Heptaminol had no action on the sodium, potassium and calcium voltage-dependent ionic conductances. 3 The hypothesis of an action via an internal alkalinization was tested by comparison with the action of NH4Cl. Both substances increased the tension. 4 The action of hepataminol was suppressed in sodium-free (TRIS) solution or in the presence of amiloride while the action of NH4Cl was always observed. 5 These results could be explained by a stimulation of the Na/H antiport by heptaminol.