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Articles 1 - 6 of 6
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Microrna Cargo Of Extracellular Vesicles From Alcohol-Exposed Monocytes Signals Naive Monocytes To Differentiate Into M2 Macrophages, Banishree Saha, Fatemeh Momen-Heravi, Karen Kodys, Gyongyi Szabo
Microrna Cargo Of Extracellular Vesicles From Alcohol-Exposed Monocytes Signals Naive Monocytes To Differentiate Into M2 Macrophages, Banishree Saha, Fatemeh Momen-Heravi, Karen Kodys, Gyongyi Szabo
Gyongyi Szabo
Membrane-coated extracellular vesicles (EVs) released by cells can serve as vehicles for delivery of biological materials and signals. Recently, we demonstrated that alcohol-treated hepatocytes cross-talk with immune cells via exosomes containing microRNA (miRNAs). Here, we hypothesized that alcohol-exposed monocytes can communicate with naive monocytes via EVs. We observed increased numbers of EVs, mostly exosomes, secreted by primary human monocytes and THP-1 monocytic cells in the presence of alcohol in a concentration- and time-dependent manner. EVs derived from alcohol-treated monocytes stimulated naive monocytes to polarize into M2 macrophages as indicated by increased surface expression of CD68 (macrophage marker), M2 markers (CD206 …
Structural Basis For Mutation-Induced Destabilization Of Profilin 1 In Als, Sivakumar Boopathy, Tania Silvas, Maeve Tischbein, Silvia Jansen, Shivender Shandilya, Jill Zitzewitz, John Landers, Bruce Goode, Celia Schiffer, Daryl Bosco
Structural Basis For Mutation-Induced Destabilization Of Profilin 1 In Als, Sivakumar Boopathy, Tania Silvas, Maeve Tischbein, Silvia Jansen, Shivender Shandilya, Jill Zitzewitz, John Landers, Bruce Goode, Celia Schiffer, Daryl Bosco
Celia A. Schiffer
Mutations in profilin 1 (PFN1) are associated with amyotrophic lateral sclerosis (ALS); however, the pathological mechanism of PFN1 in this fatal disease is unknown. We demonstrate that ALS-linked mutations severely destabilize the native conformation of PFN1 in vitro and cause accelerated turnover of the PFN1 protein in cells. This mutation-induced destabilization can account for the high propensity of ALS-linked variants to aggregate and also provides rationale for their reported loss-of-function phenotypes in cell-based assays. The source of this destabilization is illuminated by the X-ray crystal structures of several PFN1 proteins, revealing an expanded cavity near the protein core of the …
Heat Shock Proteins And Whole Body Physiology, Earl Nobe, C.W. James Melling, Kevin Milne
Heat Shock Proteins And Whole Body Physiology, Earl Nobe, C.W. James Melling, Kevin Milne
Jamie Melling
No abstract provided.
Imaging Of Transplanted Pancreatic Islets In Vivo, P. Joo Ho Tai, C. Foster, C. Hasil, C.W.J. Melling, D. White
Imaging Of Transplanted Pancreatic Islets In Vivo, P. Joo Ho Tai, C. Foster, C. Hasil, C.W.J. Melling, D. White
Jamie Melling
No abstract provided.
Imaging Islets Labeled Wth Magnetic Nanoparticules At 1.5 Tesla, J.H. Tai, Paula Foster, Alma Rosales, Biao Feng, Craig Ha, Violetta Martinez, Soha Ramadan, Jonatan Snir, C.W. Melling, Savita Dhanvantari, Brian Rutt, David White
Imaging Islets Labeled Wth Magnetic Nanoparticules At 1.5 Tesla, J.H. Tai, Paula Foster, Alma Rosales, Biao Feng, Craig Ha, Violetta Martinez, Soha Ramadan, Jonatan Snir, C.W. Melling, Savita Dhanvantari, Brian Rutt, David White
Jamie Melling
We have developed a magnetic resonance imaging (MRI) technique for imaging Feridex (superparamagnetic iron oxide [SPIO])-labeled islets of Langerhans using a standard clinical 1.5-Tesla (T) scanner and employing steady-state acquisition imaging sequence (3DFIESTA). Both porcine and rat islets were labeled with SPIO by a transfection technique using a combination of poly-l-lysine and electroporation. Electron microscopy demonstrated presence of SPIO particles within the individual islet cells, including beta-cells and particles trapped between cell membranes. Our labeling method produced a transfection rate of 860 pg to 3.4 ng iron per islet, dependent on the size of the islet. The labeling procedure did …
Regulation Of Myocardial Hsp70 Gene Expression Following Exercise, C.W. Melling, David Thorp, Earl Noble
Regulation Of Myocardial Hsp70 Gene Expression Following Exercise, C.W. Melling, David Thorp, Earl Noble
Jamie Melling
No abstract provided.