Biochemistry, Biophysics, and Structural Biology Commons^™

Netrin-3 Signals Through Serine Phosphorylation In Tetrahymena Thermophila, Cayla C. Eckley, Rebecca N. Haught, Kyle J. Hooper, Jared E. Matz, Joshua L. Wilson, Bethany C. Khol, Katelyn R. Malik, Heather G. Kuruvilla

The Research and Scholarship Symposium (2013-2019)

The netrin family of proteins are structurally related to laminin and, while first discovered in the nematode Caenorhabditis elegans, are now known to be present in species throughout the animal kingdom, including humans. These proteins also have a wide variety of roles that include inhibition of apoptosis, chemorepulsion, and axonal guidance. Due to the results of previous studies involving netrin-1 in vertebrate systems, the current prevailing assumption is that netrins, when acting as chemorepellents, signal using tyrosine kinases. However, data that we gathered through phosphoserine-targeting ELISA assays and immunofluorescence microscopy demonstrates that the netrin-3 peptides signal Tetrahymena thermophila through serine …