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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Cancellation Of Cellular Responses To Nanoelectroporation By Reversing The Stimulus Polarity, Andrei G. Pakhomov, Iurii Semenov, Shu Xiao, Olga N. Pakhomova, Betsy Gregory, Karl H. Schoenbach
Cancellation Of Cellular Responses To Nanoelectroporation By Reversing The Stimulus Polarity, Andrei G. Pakhomov, Iurii Semenov, Shu Xiao, Olga N. Pakhomova, Betsy Gregory, Karl H. Schoenbach
Bioelectrics Publications
Nanoelectroporation of biomembranes is an effect of high-voltage, nanosecond-duration electric pulses (nsEP). It occurs both in the plasma membrane and inside the cell, and nanoporated membranes are distinguished by ion-selective and potential-sensitive permeability. Here we report a novel phenomenon of bioeffects cancellation that puts nsEP cardinally apart from the conventional electroporation and electrostimulation by milli- and microsecond pulses. We compared the effects of 60- and 300-ns monopolar, nearly rectangular nsEP on intracellular Ca2+mobilization and cell survival with those of bipolar 60 + 60 and 300 + 300 ns pulses. For diverse endpoints, exposure conditions, pulse numbers (1-60), and …