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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Retinal Progenitor Cells Release Extracellular Vesicles Containing Developmental Transcription Factors, Microrna And Membrane Proteins, Jing Zhou, Alberto Benito-Martin, Jason Mighty, Lynne Chang, Shima Ghoroghi, Hao Wu, Madeline Wong, Sara Guariglia, Petr Baranov, Michael Young, Rajendra Ghardbaran, Mark Emerson, Milica Tesic Mark, Henrik Molina, M. Valeria Canto-Solar, Hector Peinado Selgas, Stephen Redenti
Retinal Progenitor Cells Release Extracellular Vesicles Containing Developmental Transcription Factors, Microrna And Membrane Proteins, Jing Zhou, Alberto Benito-Martin, Jason Mighty, Lynne Chang, Shima Ghoroghi, Hao Wu, Madeline Wong, Sara Guariglia, Petr Baranov, Michael Young, Rajendra Ghardbaran, Mark Emerson, Milica Tesic Mark, Henrik Molina, M. Valeria Canto-Solar, Hector Peinado Selgas, Stephen Redenti
Publications and Research
A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP. Proteomic analysis of EV content revealed retinogenic …
Cloning And Spatiotemporal Expression Of Xenopus Laevis Apolipoprotein Ci, Jyotsna Sridharan, Tomomi Haremaki, Daniel C. Weinstein
Cloning And Spatiotemporal Expression Of Xenopus Laevis Apolipoprotein Ci, Jyotsna Sridharan, Tomomi Haremaki, Daniel C. Weinstein
Publications and Research
Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer’s disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest. These data suggest as yet uncharacterized roles for ApoCI during early vertebrate …
The Microbe Directory: An Annotated, Searchable Inventory Of Microbes’ Characteristics, Heba Shaaban, David A. Westfall, Rawhi Mohammad, David Danko, Daniela Bezdan, Ebrahim Afshinnekoo, Nicola Segata, Christopher E. Mason
The Microbe Directory: An Annotated, Searchable Inventory Of Microbes’ Characteristics, Heba Shaaban, David A. Westfall, Rawhi Mohammad, David Danko, Daniela Bezdan, Ebrahim Afshinnekoo, Nicola Segata, Christopher E. Mason
Publications and Research
The Microbe Directory is a collective research effort to profile and annotate more than 7,500 unique microbial species from the MetaPhlAn2 database that includes bacteria, archaea, viruses, fungi, and protozoa. By collecting and summarizing data on various microbes’ characteristics, the project comprises a database that can be used downstream of large-scale metagenomic taxonomic analyses, allowing one to interpret and explore their taxonomic classifications to have a deeper understanding of the microbial ecosystem they are studying. Such characteristics include, but are not limited to: optimal pH, optimal temperature, Gram stain, biofilm-formation, spore-formation, antimicrobial resistance, and COGEM class risk rating. The database …