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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Dynamic Surfaces For The Study Of Mesenchymal Stem Cell Growth Through Adhesion Regulation, Jemma N. Roberts, Jugal Kishore Sahoo, Laura E. Mcnamara, Karl V. Burgess, Jingli Yang, Enateri V. Alakpa, Hilary J. Anderson, Jake Hay, Lesley-Anne Turner, Stephen J. Yarwood, Mischa Zelzer, Richard O.C. Oreffo, Rein V. Ulijn, Matthew J. Dalby
Dynamic Surfaces For The Study Of Mesenchymal Stem Cell Growth Through Adhesion Regulation, Jemma N. Roberts, Jugal Kishore Sahoo, Laura E. Mcnamara, Karl V. Burgess, Jingli Yang, Enateri V. Alakpa, Hilary J. Anderson, Jake Hay, Lesley-Anne Turner, Stephen J. Yarwood, Mischa Zelzer, Richard O.C. Oreffo, Rein V. Ulijn, Matthew J. Dalby
Advanced Science Research Center
Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/ cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate …
Analysis Of Enzyme-Responsive Peptide Surfaces By Raman Spectroscopy, Jugal Kishore Sahoo, Narayana M.S. Sirimuthu, Anne Canning, Mischa Zelzer, Duncan Graham, Rein V. Ulijn
Analysis Of Enzyme-Responsive Peptide Surfaces By Raman Spectroscopy, Jugal Kishore Sahoo, Narayana M.S. Sirimuthu, Anne Canning, Mischa Zelzer, Duncan Graham, Rein V. Ulijn
Advanced Science Research Center
We report on the use of Raman spectroscopy as a tool to characterize model peptide functionalised surfaces. By taking advantage of Raman reporters built into the peptide sequence, the enzymatic hydrolysis of these peptides could be determined.