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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Quantitative And Functional Analysis Pipeline For Label-Free Metaproteomics Data And Its Applications, Lang Ho Lee
Quantitative And Functional Analysis Pipeline For Label-Free Metaproteomics Data And Its Applications, Lang Ho Lee
Doctoral Dissertations
Since the large-scale metaproteome was first reported in 2005, metaproteomics has advanced at a tremendous rate both in its quantitative and qualitative metrics. Furthermore metaproteomics is now being applied as a general tool in microbial ecology in a large variety of environmental studies. Though metaproteomics is becoming a useful and even a standard tool for the microbial ecologist, standardized bioinformatics pipelines are not readily available. Therefore, we developed quantitative and functional analysis pipeline for metaproteomics (QFAM) to help analyze large and complicated metaproteomics data in a robust and timely fashion with outputs designed to be simple and clearly understood by …
Constitutive Activity In Orphan G Protein Coupled Receptors, Adam Lee Martin
Constitutive Activity In Orphan G Protein Coupled Receptors, Adam Lee Martin
Doctoral Dissertations
"The goal of this research was to use a distal signaling pathway analysis to evaluate the extent of agonist independent constitutive signaling among orphan class-A G protein coupled receptors (GPCRs). These receptors translate extracellular signals via conformational change into intracellular activation of different G proteins and subsequent second messenger synthesis. These small molecules regulate cellular biochemistry, eventually leading to nuclear signaling that results in changes in gene expression. Some GPCRs are capable of signaling in the absence of an activating ligand, a phenomenon called constitutive activity that is inhibited via an "inverse-agonist". The use of cAMP dependent Luciferase expression is …