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- Bioprocess modeling (1)
- Brackish waters (1)
- Chromatography (1)
- DNA Repair (1)
- Double-Strand DNA Break (1)
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- Fatty acid biosynthesis (1)
- Fermentation (1)
- Glucose Transporter (1)
- Homologous Recombination (1)
- Hydrogen (1)
- Lipid biochemistry (1)
- Lipid metabolism (1)
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- Plastid biochemistry (1)
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- Reductive Dechlorination (1)
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- Soybean somatic embryo plastids (1)
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Articles 1 - 8 of 8
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Attenuation Of Human Dmc1 Recombination Activities By Substitution Of A Conserved Residue, Melody Hayman
Attenuation Of Human Dmc1 Recombination Activities By Substitution Of A Conserved Residue, Melody Hayman
All Theses
Unrepaired DNA double strand breaks (DSB) are detrimental to the viability of the cell. The repair of DSBs can occur by a process called homologous recombination (HR). HR is used whenever the cell has a homologous template in the way of a homologous chromosome available. The process of HR during mitosis is different from meiotic HR. To initiate meiotic recombination, DSBs are formed and processed to allow proper chromosome segregation and ultimately genetic variation. Meiotic HR is mediated by the meiosis specific recombinase, DMC1. The DMC1 recombinase binds to the 3’ end of a newly resected DSB forming a nucleoprotein …
Investigating The Biochemical Properties Of A Novel Mutation, A194v, In Human Rad51, Briana Vollbeer
Investigating The Biochemical Properties Of A Novel Mutation, A194v, In Human Rad51, Briana Vollbeer
All Theses
DNA double-strand breaks (DSB) are one of the most serious DNA lesions because improper repair of a DSB can lead to loss of heterozygosity, aneuploidy, and cancer. One of the primary pathways to repair DSBs is homologous recombination (HR). HR resects the DNA around the DSB and then uses homologous DNA as a template to restore the broken sequence. RAD51 has a vital function in this pathway by forming a nucleoprotein filament on a resected end of the DSB. The nucleoprotein filament searches for homology within the homologous DNA. Once homology is located, strand invasion followed by strand exchange occurs. …
Characterization Of A Potential Glucose Transporter In Trypanosoma Brucei, Matthew Morgan
Characterization Of A Potential Glucose Transporter In Trypanosoma Brucei, Matthew Morgan
All Theses
Trypanosoma brucei, the African trypanosome, is an organism heavily dependent on glucose for ATP production during the infectious stage of its life cycle. Here, we have explored the role of an uncharacterized protein designated “novel glucose transporter” (NGT) as a potential glucose transporter. Sequence analyses suggests that NGT shares similarities (either at the primary sequence level or structurally) with Trypanosome Hexose Transporters 1 (TbTHT1), and human GLUT3, both of which are membrane sugar transporters. NGT was localized by fluorescence microscopy to subcellular structures consistent with lysosomes. Silencing NGT expression with RNA interference in parasites resulted in a growth defect …
Floating Treatment Wetlands For Brackish Waters: Plant Selection And Nutrient Uptake Potential., Andrea Landaverde
Floating Treatment Wetlands For Brackish Waters: Plant Selection And Nutrient Uptake Potential., Andrea Landaverde
All Theses
Brackish water bodies in coastal regions provide critical ecosystem services that support human and environmental health. Anthropogenic activities such as agricultural and industrial activities, construction, urban settlements, and tourism contribute to increased inputs of nitrogen (N) and phosphorus (P) in brackish coastal ecosystems. Excess nutrients can lead to impaired water quality and affect marine organisms. Floating treatment wetlands (FTWs) are a vegetated-base technology used to remove contaminants from water column, that has been mainly studied and applied in freshwater systems. Application of FTWs in brackish systems requires further investigation, as high salinity in brackish waters could result in toxicity to …
Development And Evaluation Of An Enrichment Culture For Reductive Dechlorination Of Tetrachloroethene Under Low Ph Conditions, Rui Xiao
All Theses
Perchloroethene (PCE) is a pollutant of major environmental concern at hazardous waste sites worldwide. PCE and trichloroethene (TCE) are suspected carcinogens and are ranked 16th and 31st, respectively, on the Environmental Protection Agency's priority list for hazardous substances, developed under the Comprehensive Environmental Response, Compensation, and Liability Act. As a consequence of the widespread use of chlorinated solvents (including PCE and TCE) for dry cleaning, chemical feedstocks, metal degreasing and other purposes, chloroethenes are widely distributed in the environment. Many soils and groundwater throughout the world are contaminated by chloroethenes. Therefore, further improvements are needed in clean-up methods. Bioaugmentation has …
Determination Of Pore Size Distribution In Capillary-Channeled Polymer (C-Cp) Fiber Stationary Phases By Inverse Size-Exclusion Chromatography (Isec) And The Study Of The Role Of Interstitial Fraction On C-Cp Fibers On Protein Binding Capacity, Zhengxin Wang
All Theses
ABSTRACT High performance liquid chromatography (HPLC), first used in the 1960's, is a rapidly evolving analytical technique, widely employed for identification, separation, and purification in biotechnology and pharmaceutical industries. The development of the stationary phases has played an important role in improving this technique. Each stationary phase will have its own disadvantages. Polysaccharide-based stationary phases such as cross-linked dextran cannot tolerate high pressures and linear velocities; silica stationary phases are rigid enough but slow mass transfer in the pores on the surface causes another problem; with the introduction of non-porous and small bead packing materials, the low surface area and …
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
All Theses
A method for the isolation of intact physiologically active plastids from rapidly developing soybean (Glycine max L.) somatic embryos has been developed for the in vitro study of lipid metabolism. Using de novo fatty acid biosynthesis from 14C-acetate as a marker for physiological functionality, the greatest rates of fatty acid biosynthesis were recovered in 3000 x g fractions that were isolated in the presence of 0.5 M sorbitol, with essentially no activity occurring in the 3000 x g supernatant. Plastids purified on 10% Percoll were approximately 70 and 97 % free from mitochondrial and ER contamination, respectively, as judged …
Biomass V2.0: A New Tool For Bioprocess Simulation, Y Phan-Thien
Biomass V2.0: A New Tool For Bioprocess Simulation, Y Phan-Thien
All Theses
A simulation software (BioMASS - Biological Modeling and Simulation Software) was upgraded from the previous version and provided with additional enhancements. Several new bioprocess configurations and their subroutines have been added. The additional processes included continuous stirred tank reactor (CSTR) with biomass recycle, and CSTR with additional stream in the second stage. The primary goal in the development of BioMASS v2.0 was to provide users with a ready-to-use, expressive visual modeling tools. In this new version, output from simulation can be visualized in graphics. Printing, exporting, and saving file options also are available. In summary, BioMASS v2.0 offers an effective …