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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Protein Engineering In The Study Of Protein Labeling And Degradation, Xinyi Zhang
Protein Engineering In The Study Of Protein Labeling And Degradation, Xinyi Zhang
Theses and Dissertations--Chemistry
Proteins are large macromolecules that play important roles in nature. With the development of modern molecular biology techniques, protein engineering has emerged as a useful tool and found many applications in areas ranging from food industry, environmental protection, to medical and life science. Biomimetic membrane incorporates biological elements, such as proteins, to form membranes that mimic the high specificity and conductance of natural biological membranes. For any application involving the usage of proteins, the first barrier is always the production of proteins with sufficient stability, and the incorporation of proteins into the artificial matrix. This thesis contains two major parts, …
Illuminate The Pathway Of Membrane Protein Association And Degradation, Zhaoshuai Wang
Illuminate The Pathway Of Membrane Protein Association And Degradation, Zhaoshuai Wang
Theses and Dissertations--Chemistry
Escherichia coli transporter protein AcrB and its homologues are the inner membrane components of the Resistance-Nodulation-Division (RND) family efflux pumps in Gram-negative bacteria. It is well accepted that soluble proteins are only marginally stable, but such insight is missing for membrane proteins. The lack of stability data, including thermodynamic stability and oligomer association affinity is a result of intrinsic difficulties in working with membrane proteins. In addition, the degradation of soluble proteins in E. coli has been extensively studied whereas the degradation process of membrane proteins remains unclear. A focus of my thesis is the validation and development of methods …
Assembly And Degradation Of A Trimeric Membrane Protein Acrb, Qian Chai
Assembly And Degradation Of A Trimeric Membrane Protein Acrb, Qian Chai
Theses and Dissertations--Chemistry
Multidrug efflux pumps are membrane proteins that actively transport foreign objects out of cells. The active efflux of these pumps is a critical self-defense mechanism that enables the survival of bacteria under hostile environments. Efflux pump AcrB is a member of the Resistance-Nodulation-Division (RND) super family. In E. coli, it associates with periplasmic protein AcrA and outer membrane channel TolC to extrude a variety of noxious compounds out of cell from both the cytoplasm and the periplasm. My dissertation research focused on two aspects of this multidrug efflux pump: the oligomerization process during the biogenesis of AcrB and its …