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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Biochemistry

California Polytechnic State University, San Luis Obispo

STAR Program Research Presentations

2015

Articles 1 - 3 of 3

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Fatty Acid Recovery And Identification In Mars Analogue Soil Samples, Kimberly E. Lykens Aug 2015

Fatty Acid Recovery And Identification In Mars Analogue Soil Samples, Kimberly E. Lykens

STAR Program Research Presentations

A primary goal of NASA’s Mars 2020 mission is to gather and store samples of Mars soil that could possibly be returned to Earth in a future mission for investigations into past or present life. In years leading up to a potential sample return mission on Mars and potentially other worlds, samples taken from Chile’s Atacama Desert, the most arid, biologically limited desert in the world, are valuable in developing a capacity for biosignature detection, specifically when exploring fatty acid abundance. Eighteen samples were collected from two sites in the Atacama characterized by biological soil crusts (BSC). BSCs are areas …


Bacterial Expression Of Plant Pyruvate Kinase, Kristina Fuller Jan 2015

Bacterial Expression Of Plant Pyruvate Kinase, Kristina Fuller

STAR Program Research Presentations

Pyruvate kinases serve an important role in regulating glycolysis pathways. Different pyruvate kinase isozymes can dictate which of several different products will be formed during the final step of glycolysis. Being able to reliably copy active pyruvate kinases (PK) would allow study of the different isozymes, with the goal of eventually creating plants that are more nutritionally beneficial for humans. This study focused on improving the protocol for replicating each of two isolated PK isozymes from the model plant Arabidopsis thaliana. Previous attempts had resulted in the PK denaturing and consequently precipitating out of solution and so we manipulated the …


Bacterial Expression Of Plant Pyruvate Kinase, Kristina Fuller Jan 2015

Bacterial Expression Of Plant Pyruvate Kinase, Kristina Fuller

STAR Program Research Presentations

Pyruvate kinases serve an important role in regulating glycolysis pathways. Different pyruvate kinase isozymes can dictate which of several different products will be formed during the final step of glycolysis. Being able to reliably copy active pyruvate kinases (PK) would allow study of the different isozymes, with the goal of eventually creating plants that are more nutritionally beneficial for humans. This study focused on improving the protocol for replicating each of two isolated PK isozymes from the model plant Arabidopsis thaliana. Previous attempts had resulted in the PK denaturing and consequently precipitating out of solution and so we manipulated the …