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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Repeated Stressors In Adulthood Increase The Rate Of Biological Ageing, Michaela Hau, Mark F. Haussmann, Timothy J. Greives, Christa Matlack, David Costantini, Michael Quetting, James S. Adelman, Ana C. Miranda, Jesko Partecke
Repeated Stressors In Adulthood Increase The Rate Of Biological Ageing, Michaela Hau, Mark F. Haussmann, Timothy J. Greives, Christa Matlack, David Costantini, Michael Quetting, James S. Adelman, Ana C. Miranda, Jesko Partecke
James S. Adelman
Individuals of the same age can differ substantially in the degree to which they have accumulated tissue damage, akin to bodily wear and tear, from past experiences. This accumulated tissue damage reflects the individual’s biological age and may better predict physiological and behavioural performance than the individual‘s chronological age. However, at present it remains unclear how to reliably assess biological age in individual wild vertebrates. We exposed hand-raised adult Eurasian blackbirds (Turdus merula) to a combination of repeated immune and disturbance stressors for over one year to determine the effects of chronic stress on potential biomarkers of biological ageing including …
Phylogeographic Analysis Of The Threatened And Endangered Superconglutinate-Producing Mussels Of The Genus Lampsilis (Bivalvia: Unionidae), Kevin J. Roe, Charles Lydeard, Paul D. Hartfield
Phylogeographic Analysis Of The Threatened And Endangered Superconglutinate-Producing Mussels Of The Genus Lampsilis (Bivalvia: Unionidae), Kevin J. Roe, Charles Lydeard, Paul D. Hartfield
Kevin J. Roe
Several species of freshwater unionid mussels in the genus Lampsilis exhibit a remarkable reproductive strategy. Female mussels of these species enclose their larvae in a minnow-like lure, called a ‘superconglutinate’, to attract piscivorous fishes. When a fish attempts to ingest the superconglutinate the lure ruptures and the larvae are released to parasitize the fish. Of the four species of mussel which exhibit this strategy and are endemic to the Gulf Coast drainages of the southeastern United States, three are protected under the Endangered Species Act, and one is recognized as imperilled. Phylogenetic analysis of nucleotide sequences of the mitochondrial 16S …
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Steven M. Lonergan
An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately 130 kDa in prerigor skeletal muscle extracts. The intensity of the immunoreactive bands corresponds qualitatively with assayable calpastatin activity. For ELISA development, optimum dilutions of sample, primary anti-calpastatin antibody, and peroxidase-conjugated secondary antibody were determined by titration. A dilution optimum for coating of Immulonâ 4 (Dynatech) plates was observed …