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Full-Text Articles in Animal Sciences
Phylogrouping And Antimicrobial Resistance Analysis Of Extraintestinal Pathogenic Escherichia Coli Isolated From Poultry Species, Sujata Bhave, Rahul Kolhe, Ranjan Mahadevaswamy, Chandrakant Bhong, Samir Jadhav, Saba Nalband, Dattatray Gandhale, Dushyant Muglikar
Phylogrouping And Antimicrobial Resistance Analysis Of Extraintestinal Pathogenic Escherichia Coli Isolated From Poultry Species, Sujata Bhave, Rahul Kolhe, Ranjan Mahadevaswamy, Chandrakant Bhong, Samir Jadhav, Saba Nalband, Dattatray Gandhale, Dushyant Muglikar
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenic Escherichia coli (ExPEC) in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened by PCR for detection of ExPEC strains. Nineteen (13.01%) isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealed as follows: broiler (25%), layer (15.87%), and raw chicken (3.12%). Turkey, duck, and water samples were negative for ExPEC strains. ExPEC strains belonged to phylogenetic groups B2 (52.63%), A (36.84%), and D (10.53%). Twenty-three (15.75%) isolates were ESBL-positive, including four ExPEC strains. ESBL-positive …
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
Turkish Journal of Veterinary & Animal Sciences
Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …