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Full-Text Articles in Animal Sciences
Application Of Lc-Ms/Ms In Identification Of Toxin In The Causative Gastropod And Victim Samples, Chun-Lan Lin, Cheng-Hong Hsieh, You-Liang Hsieh, Yi-Cheng Tai, Deng-Fwu Hwang
Application Of Lc-Ms/Ms In Identification Of Toxin In The Causative Gastropod And Victim Samples, Chun-Lan Lin, Cheng-Hong Hsieh, You-Liang Hsieh, Yi-Cheng Tai, Deng-Fwu Hwang
Journal of Marine Science and Technology
Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 µg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 …
Identification Of Causative Fish For A Food Poisioning In Taiwan By Using Sds-Page Technique, Tai-Yuan Chen, Nan-Hong Chen, Wen-Feng Lin, Ken-Lin Hwang, Yun-Chieh Huang, Deng-Fwu Hwang
Identification Of Causative Fish For A Food Poisioning In Taiwan By Using Sds-Page Technique, Tai-Yuan Chen, Nan-Hong Chen, Wen-Feng Lin, Ken-Lin Hwang, Yun-Chieh Huang, Deng-Fwu Hwang
Journal of Marine Science and Technology
A food poisoning incident has occurred due to ingestion of red grouper meat on February 2006 in southern Taiwan. Two patients showed diarrhea, muscle weakness, vertigo and severe muscle pain. The 2 pieces of causative residues were investigated by toxicity assay. In order to identify the fish species of causative samples, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique was applied in this study. According to the low molecular weight region (＜30.0 kD) of species-specific patterns extracted from both myofibrillar and sarcoplasmic proteins, two poisoning samples were identified as Lutjanus bohar. L. bohar is a notorious grouper species with ciguatera in …